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dc.contributor.authorLee, Byeong-Chel
dc.contributor.authorJiang, Shuxian
dc.contributor.authorFu, Yigong
dc.contributor.authorAvraham, Shalom
dc.contributor.authorLim, Bing
dc.contributor.authorAvraham, Hava
dc.date.accessioned2011-03-23T00:43:32Z
dc.date.issued2010
dc.identifier.citationJiang, Shuxian, Byeong-Chel Lee, Yigong Fu, Shalom Avraham, Bing Lim, and Hava Karsenty Avraham. 2010. Reconstitution of mammary epithelial morphogenesis by murine embryonic stem cells undergoing hematopoietic stem cell differentiation. PLoS ONE 5(3): e9707.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4768786
dc.description.abstractBackground: Mammary stem cells are maintained within specific microenvironments and recruited throughout lifetime to reconstitute de novo the mammary gland. Mammary stem cells have been isolated through the identification of specific cell surface markers and in vivo transplantation into cleared mammary fat pads. Accumulating evidence showed that during the reformation of mammary stem cell niches by dispersed epithelial cells in the context of the intact epithelium-free mammary stroma, non-mammary epithelial cells may be sequestered and reprogrammed to perform mammary epithelial cell functions and to adopt mammary epithelial characteristics during reconstruction of mammary epithelium in regenerating mammary tissue in vivo. Methodology/Principal Findings: To examine whether other types of progenitor cells are able to contribute to mammary branching morphogenesis, we examined the potential of murine embryonic stem (mES) cells, undergoing hematopoietic differentiation, to support mammary reconstitution in vivo. We observed that cells from day 14 embryoid bodies (EBs) under hematopoietic differentiation condition, but not supernatants derived from these cells, when transplanted into denuded mammary fat pads, were able to contribute to both the luminal and myoepithelial lineages in branching ductal structures resembling the ductal-alveolar architecture of the mammary tree. No teratomas were observed when these cells were transplanted in vivo. Conclusions/Significance: Our data provide evidence for the dominance of the tissue-specific mammary stem cell niche and its role in directing mES cells, undergoing hematopoietic differentiation, to reprogram into mammary epithelial cells and to promote mammary epithelial morphogenesis. These studies should also provide insights into regeneration of damaged mammary gland and the role of the mammary microenvironment in reprogramming cell fate.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0009707en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837751/pdf/en_US
dash.licenseLAA
dc.subjectdevelopmental biologyen_US
dc.subjectstem cellsen_US
dc.subjectoncologyen_US
dc.subjectbreast canceren_US
dc.subjectwomen's healthen_US
dc.titleReconstitution of Mammary Epithelial Morphogenesis by Murine Embryonic Stem Cells Undergoing Hematopoietic Stem Cell Differentiationen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorAvraham, Shalom
dc.date.available2011-03-23T00:43:32Z
dash.affiliation.otherHMS^Medicine- Beth Israel-Deaconessen_US
dash.affiliation.otherHMS^Medicine- Beth Israel-Deaconessen_US
dc.identifier.doi10.1371/journal.pone.0009707*
dash.authorsorderedfalse
dash.contributor.affiliatedLim, Bing
dash.contributor.affiliatedJiang, Shuxian
dash.contributor.affiliatedAvraham, Shalom
dash.contributor.affiliatedAvraham, Hava
dash.contributor.affiliatedFu, Yigong


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