mRNA Turnover Rate Limits siRNA and microRNA Efficacy

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mRNA Turnover Rate Limits siRNA and microRNA Efficacy

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Title: mRNA Turnover Rate Limits siRNA and microRNA Efficacy
Author: Larsson, Erik; Sander, Chris; Marks, Debora

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Citation: Larsson, Erik, Chris Sander, and Debora Marks. 2010. mRNA turnover rate limits siRNA and microRNA efficacy. Molecular Systems Biology 6: 433.
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Abstract: Based on a simple model of the mRNA life cycle, we predict that mRNAs with high turnover rates in the cell are more difficult to perturb with RNAi. We test this hypothesis using a luciferase reporter system and obtain additional evidence from a variety of large-scale data sets, including microRNA overexpression experiments and RT–qPCR-based efficacy measurements for thousands of siRNAs. Our results suggest that mRNA half-lives will influence how mRNAs are differentially perturbed whenever small RNA levels change in the cell, not only after transfection but also during differentiation, pathogenesis and normal cell physiology.
Published Version: doi:10.1038/msb.2010.89
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