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dc.contributor.authorLarsson, Erik
dc.contributor.authorSander, Chris
dc.contributor.authorMarks, Debora
dc.date.accessioned2011-04-11T14:42:47Z
dc.date.issued2010
dc.identifier.citationLarsson, Erik, Chris Sander, and Debora Marks. 2010. mRNA turnover rate limits siRNA and microRNA efficacy. Molecular Systems Biology 6: 433.en_US
dc.identifier.issn1744-4292en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4820784
dc.description.abstractBased on a simple model of the mRNA life cycle, we predict that mRNAs with high turnover rates in the cell are more difficult to perturb with RNAi. We test this hypothesis using a luciferase reporter system and obtain additional evidence from a variety of large-scale data sets, including microRNA overexpression experiments and RT–qPCR-based efficacy measurements for thousands of siRNAs. Our results suggest that mRNA half-lives will influence how mRNAs are differentially perturbed whenever small RNA levels change in the cell, not only after transfection but also during differentiation, pathogenesis and normal cell physiology.en_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofdoi:10.1038/msb.2010.89en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010119/pdf/en_US
dash.licenseLAA
dc.subjectmicroRNAen_US
dc.subjectmRNA decayen_US
dc.subjectRNAien_US
dc.subjectsiRNAen_US
dc.titlemRNA Turnover Rate Limits siRNA and microRNA Efficacyen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalMolecular Systems Biologyen_US
dash.depositing.authorMarks, Debora
dc.date.available2011-04-11T14:42:47Z
dash.affiliation.otherHMS^Systems Biologyen_US
dc.identifier.doi10.1038/msb.2010.89*
dash.contributor.affiliatedMarks, Debora


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