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dc.contributor.authorKim, Douglas S
dc.contributor.authorRoss, Sarah Elizabeth
dc.contributor.authorTrimarchi, Jeffrey M
dc.contributor.authorAach, John Dennis
dc.contributor.authorGreenberg, Michael Eldon
dc.contributor.authorCepko, Connie
dc.date.accessioned2011-04-16T01:49:03Z
dc.date.issued2008
dc.identifier.citationKim, Douglas S., Sarah E. Ross, Jeffrey M. Trimarchi, John Aach, Michael E. Greenberg, and Constance L. Cepko. 2008. Identification of molecular markers of bipolar cells in the murine retina. The Journal of Comparative Neurology 507(5): 1795-1810.en_US
dc.identifier.issn0021-9967en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4851330
dc.description.abstractRetinal bipolar neurons serve as relay interneurons that connect rod and cone photoreceptor cells to amacrine and ganglion cells. They exhibit diverse morphologies essential for correct routing of photoreceptor cell signals to specific postsynaptic amacrine and ganglion cells. The development and physiology of these interneurons have not been completely defined molecularly. Despite previous identification of genes expressed in several bipolar cell subtypes, molecules that mark each bipolar cell type still await discovery. In this report, novel genetic markers of murine bipolar cells were found. Candidates were initially generated by using microarray analysis of single bipolar cells and mining of retinal serial analysis of gene expression (SAGE) data. These candidates were subsequently tested for expression in bipolar cells by RNA in situ hybridization. Ten new molecular markers were identified, five of which are highly enriched in their expression in bipolar cells within the adult retina. Double-labeling experiments using probes for previously characterized subsets of bipolar cells were performed to identify the subtypes of bipolar cells that express the novel markers. Additionally, the expression of bipolar cell genes was analyzed in Bhlhb4 knockout retinas, in which rod bipolar cells degenerate postnatally, to delineate further the identity of bipolar cells in which novel markers are found. From the analysis of Bhlhb4 mutant retinas, cone bipolar cell gene expression appears to be relatively unaffected by the degeneration of rod bipolar cells. Identification of molecular markers for the various subtypes of bipolar cells will lead to greater insights into the development and function of these diverse interneurons.en_US
dc.language.isoen_USen_US
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.relation.isversionofdoi:10.1002/cne.21639en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665264/pdf/en_US
dash.licenseLAA
dc.subjectretinaen_US
dc.subjectbipolar cellsen_US
dc.subjectBhlhb4en_US
dc.subjectgene expressionen_US
dc.subjectmicroarrayen_US
dc.subjectmouseen_US
dc.titleIdentification of Molecular Markers of Bipolar Cells in the Murine Retinaen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalThe Journal of Comparative Neurologyen_US
dash.depositing.authorAach, John Dennis
dc.date.available2011-04-16T01:49:03Z
dash.affiliation.otherHMS^Geneticsen_US
dash.affiliation.otherHMS^Ophthalmologyen_US
dash.affiliation.otherHMS^Geneticsen_US
dc.identifier.doi10.1002/cne.21639*
dash.contributor.affiliatedRoss, Sarah Elizabeth
dash.contributor.affiliatedAach, John
dash.contributor.affiliatedGreenberg, Michael
dash.contributor.affiliatedCepko, Constance


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