Identification of Molecular Markers of Bipolar Cells in the Murine Retina

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Identification of Molecular Markers of Bipolar Cells in the Murine Retina

Show simple item record Kim, Douglas S Trimarchi, Jeffrey M Ross, Sarah Elizabeth Aach, John Dennis Greenberg, Michael Eldon Cepko, Connie 2011-04-16T01:49:03Z 2008
dc.identifier.citation Kim, Douglas S., Sarah E. Ross, Jeffrey M. Trimarchi, John Aach, Michael E. Greenberg, and Constance L. Cepko. 2008. Identification of molecular markers of bipolar cells in the murine retina. The Journal of Comparative Neurology 507(5): 1795-1810. en_US
dc.identifier.issn 0021-9967 en_US
dc.description.abstract Retinal bipolar neurons serve as relay interneurons that connect rod and cone photoreceptor cells to amacrine and ganglion cells. They exhibit diverse morphologies essential for correct routing of photoreceptor cell signals to specific postsynaptic amacrine and ganglion cells. The development and physiology of these interneurons have not been completely defined molecularly. Despite previous identification of genes expressed in several bipolar cell subtypes, molecules that mark each bipolar cell type still await discovery. In this report, novel genetic markers of murine bipolar cells were found. Candidates were initially generated by using microarray analysis of single bipolar cells and mining of retinal serial analysis of gene expression (SAGE) data. These candidates were subsequently tested for expression in bipolar cells by RNA in situ hybridization. Ten new molecular markers were identified, five of which are highly enriched in their expression in bipolar cells within the adult retina. Double-labeling experiments using probes for previously characterized subsets of bipolar cells were performed to identify the subtypes of bipolar cells that express the novel markers. Additionally, the expression of bipolar cell genes was analyzed in Bhlhb4 knockout retinas, in which rod bipolar cells degenerate postnatally, to delineate further the identity of bipolar cells in which novel markers are found. From the analysis of Bhlhb4 mutant retinas, cone bipolar cell gene expression appears to be relatively unaffected by the degeneration of rod bipolar cells. Identification of molecular markers for the various subtypes of bipolar cells will lead to greater insights into the development and function of these diverse interneurons. en_US
dc.language.iso en_US en_US
dc.publisher Wiley Subscription Services, Inc., A Wiley Company en_US
dc.relation.isversionof doi:10.1002/cne.21639 en_US
dc.relation.hasversion en_US
dash.license LAA
dc.subject retina en_US
dc.subject bipolar cells en_US
dc.subject Bhlhb4 en_US
dc.subject gene expression en_US
dc.subject microarray en_US
dc.subject mouse en_US
dc.title Identification of Molecular Markers of Bipolar Cells in the Murine Retina en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal The Journal of Comparative Neurology en_US Aach, John Dennis 2011-04-16T01:49:03Z
dash.affiliation.other HMS^Genetics en_US
dash.affiliation.other HMS^Ophthalmology en_US
dash.affiliation.other HMS^Genetics en_US

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