Show simple item record

dc.contributor.authorBlackshaw, Seth
dc.contributor.authorKuo, Winston Patrick
dc.contributor.authorPark, Peter J.
dc.contributor.authorTsujikawa, Motokazu
dc.contributor.authorGunnersen, Jenny M
dc.contributor.authorScott, Hamish S
dc.contributor.authorBoon, Wee-Ming
dc.contributor.authorTan, Seong-Seng
dc.contributor.authorCepko, Constance L.
dc.date.accessioned2011-04-20T19:31:42Z
dc.date.issued2003
dc.identifier.citationBlackshaw, Seth, Winston P. Kuo, Peter J. Park, Motokazu Tsujikawa, Jenny M. Gunnersen, Hamish S. Scott, Wee-Ming Boon, Seong-Seng Tan, and Constance L. Cepko. 2003. MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues. Genome Biology 4(3): R17.en_US
dc.identifier.issn1465-6906en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4870973
dc.description.abstractBackground: Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate. Results: We show that microSAGE is highly representative, reproducible and accurate, but that pronounced differences in gene expression are seen between tissue samples taken from different individuals. Conclusions: MicroSAGE is a reliable method of comprehensively profiling differences in gene expression among samples, but care should be taken in generalizing results obtained from libraries constructed from tissue obtained from different individuals and/or processed or stored differently.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC153457/pdf/en_US
dash.licenseLAA
dc.titleMicroSAGE is Highly Representative and Reproducible but Reveals Major Differences in Gene Expression Among Samples Obtained from Similar Tissuesen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalGenome Biologyen_US
dash.depositing.authorPark, Peter J.
dc.date.available2011-04-20T19:31:42Z
dash.affiliation.otherHMS^Health Sciences and Technologyen_US
dash.affiliation.otherHMS^Pediatrics-Children's Hospitalen_US
dash.affiliation.otherHMS^Ophthalmologyen_US
dash.affiliation.otherHMS^Geneticsen_US
dash.contributor.affiliatedKuo, Winston
dash.contributor.affiliatedPark, Peter
dash.contributor.affiliatedCepko, Constance


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record