A Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensis

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Author
Murthy, Tal
Raphael, Jacob
Moreira, Donna
Kelley, Fontina
Taycher, Elena
Brizuela, Leonardo
LaBaer, Joshua
Petrosino, Joseph
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1371/journal.pone.0000577Metadata
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Murthy, Tal, Andreas Rolfs, Yanhui Hu, Zhenwei Shi, Jacob Raphael, Donna Moreira, Fontina Kelley, et al. 2007. A full-genomic sequence-verified protein-coding gene collection for Francisella tularensis. PLoS ONE 2(6): e577.Abstract
The rapid development of new technologies for the high throughput (HT) study of proteins has increased the demand for comprehensive plasmid clone resources that support protein expression. These clones must be full-length, sequence-verified and in a flexible format. The generation of these resources requires automated pipelines supported by software management systems. Although the availability of clone resources is growing, current collections are either not complete or not fully sequence-verified. We report an automated pipeline, supported by several software applications that enabled the construction of the first comprehensive sequence-verified plasmid clone resource for more than 96% of protein coding sequences of the genome of F. tularensis, a highly virulent human pathogen and the causative agent of tularemia. This clone resource was applied to a HT protein purification pipeline successfully producing recombinant proteins for 72% of the genes. These methods and resources represent significant technological steps towards exploiting the genomic information of F. tularensis in discovery applications.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894649/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:4874788
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