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dc.contributor.authorMurthy, Tal
dc.contributor.authorRaphael, Jacob
dc.contributor.authorMoreira, Donna
dc.contributor.authorKelley, Fontina
dc.contributor.authorTaycher, Elena
dc.contributor.authorBrizuela, Leonardo
dc.contributor.authorLaBaer, Joshua
dc.contributor.authorPetrosino, Joseph
dc.contributor.authorRolfs, Andreas
dc.contributor.authorHu, Yanhui
dc.contributor.authorMcCarron, Seamus
dc.contributor.authorZuo, Dongmei
dc.contributor.authorShi, Zhenwei
dc.contributor.authorJepson, Daniel Ashley
dc.contributor.authorMohr, Stephanie
dc.contributor.authorFernandez, Mauricio J.
dc.date.accessioned2011-04-23T14:37:09Z
dc.date.issued2007
dc.identifier.citationMurthy, Tal, Andreas Rolfs, Yanhui Hu, Zhenwei Shi, Jacob Raphael, Donna Moreira, Fontina Kelley, et al. 2007. A full-genomic sequence-verified protein-coding gene collection for Francisella tularensis. PLoS ONE 2(6): e577.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4874788
dc.description.abstractThe rapid development of new technologies for the high throughput (HT) study of proteins has increased the demand for comprehensive plasmid clone resources that support protein expression. These clones must be full-length, sequence-verified and in a flexible format. The generation of these resources requires automated pipelines supported by software management systems. Although the availability of clone resources is growing, current collections are either not complete or not fully sequence-verified. We report an automated pipeline, supported by several software applications that enabled the construction of the first comprehensive sequence-verified plasmid clone resource for more than 96% of protein coding sequences of the genome of F. tularensis, a highly virulent human pathogen and the causative agent of tularemia. This clone resource was applied to a HT protein purification pipeline successfully producing recombinant proteins for 72% of the genes. These methods and resources represent significant technological steps towards exploiting the genomic information of F. tularensis in discovery applications.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0000577en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894649/pdf/en_US
dash.licenseLAA
dc.subjectinfectious diseasesen_US
dc.subjectmicrobiologyen_US
dc.subjectbiotechnologyen_US
dc.subjectprotein chemistry and proteomicsen_US
dc.titleA Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensisen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorMohr, Stephanie
dc.date.available2011-04-23T14:37:09Z
dash.affiliation.otherHMS^Biological Chemistry and Molecular Pharmacologyen_US
dash.affiliation.otherHMS^Biological Chemistry and Molecular Pharmacologyen_US
dash.affiliation.otherHMS^Pathologyen_US
dash.affiliation.otherHMS^Geneticsen_US
dc.identifier.doi10.1371/journal.pone.0000577*
dash.authorsorderedfalse
dash.contributor.affiliatedMcCarron, Seamus
dash.contributor.affiliatedShi, Zhenwei
dash.contributor.affiliatedFernandez, Mauricio
dash.contributor.affiliatedJepson, Daniel
dash.contributor.affiliatedRolfs, Andreas
dash.contributor.affiliatedZuo, Dongmei
dash.contributor.affiliatedMohr, Stephanie
dash.contributor.affiliatedHu, Yanhui


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