dc.contributor.author | Yin, Hong | |
dc.contributor.author | Radomska, Hanna S. | |
dc.contributor.author | Tenen, Daniel Geoffrey | |
dc.contributor.author | Glass, Jonathan | |
dc.date.accessioned | 2011-04-24T22:01:47Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Yin, Hong, Hanna S. Radomska, Daniel G. Tenen, and Jonathan Glass. 2006. Down regulation of PSA by C/EBPα is associated with loss of AR expression and inhibition of PSA promoter activity in the LNCaP cell Line. BMC Cancer 6: 158. | en_US |
dc.identifier.issn | 1471-2407 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4874840 | |
dc.description.abstract | Background: C/EBPα is a transcription factor essential for terminal differentiation of several cell types. It has not known if C/EBPα protein is expressed and functions in the prostate gland. Methods: The presence of C/EBPα in normal and cancerous prostate epithelium was examined by immunochemistry. Over expression of C/EBPα in LNCaP cells was conducted with retrovirus-mediated transduction. PSA expression was examined by RT-PCR and western blot and PSA promoter activity by luciferase reporter assay. Results: In normal prostate C/EBPα was expressed in the basal layer of the epithelium. In prostate cancer C/EBPα was detected at low levels throughout the cancers and in advanced prostate cancer C/EBPα expression was associated with decreased expression of AR and PSA. Overexpression of C/EBPα inhibited epigenetically PSA expression and was accompanied by the loss of expression of AR. Transient increase of C/EBPα inhibited the PSA promoter/enhancer activity independently of expression of AR. Conclusion: In LNCaP cells C/EBPα over expression inhibits expression of PSA by AR -dependent and independent mechanisms and by extinguishing AR expression provides a model for hormonal independent cell growth. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | BioMed Central | en_US |
dc.relation.isversionof | doi:10.1186/1471-2407-6-158 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1544346/pdf/ | en_US |
dash.license | LAA | |
dc.title | Down regulation of PSA by C/EBPα is associated with loss of AR expression and inhibition of PSA promoter activity in the LNCaP cell Line | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | BMC Cancer | en_US |
dash.depositing.author | Yin, Hong | |
dc.date.available | 2011-04-24T22:01:47Z | |
dash.affiliation.other | HMS^Medicine- Beth Israel-Deaconess | en_US |
dash.affiliation.other | HMS^Medicine- Beth Israel-Deaconess | en_US |
dc.identifier.doi | 10.1186/1471-2407-6-158 | * |
dash.contributor.affiliated | Yin, Hong | |
dash.contributor.affiliated | Radomska, Hanna S. | |
dash.contributor.affiliated | Tenen, Daniel | |