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dc.contributor.authorSloan, Kevin E
dc.contributor.authorEustace, Brenda K
dc.contributor.authorStewart, Jean K
dc.contributor.authorZehetmeier, Carol
dc.contributor.authorTorella, Claudia
dc.contributor.authorSimeone, Marina
dc.contributor.authorRoy, Jennifer E
dc.contributor.authorUnger, Christine
dc.contributor.authorIlag, Leodevico L
dc.contributor.authorJay, Daniel G
dc.contributor.authorLouis, David Neil
dc.date.accessioned2011-04-26T01:44:00Z
dc.date.issued2004
dc.identifier.citationSloan, Kevin E., Brenda K. Eustace, Jean K. Stewart, Carol Zehetmeier, Claudia Torella, Marina Simeone, Jennifer E. Roy, et al. 2004. CD155/PVR plays a key role in cell motility during tumor cell invasion and migration. BMC Cancer 4: 73.en_US
dc.identifier.issn1471-2407en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4875887
dc.description.abstractBackground: Invasion is an important early step of cancer metastasis that is not well understood. Developing therapeutics to limit metastasis requires the identification and validation of candidate proteins necessary for invasion and migration. Methods: We developed a functional proteomic screen to identify mediators of tumor cell invasion. This screen couples Fluorophore Assisted Light Inactivation (FALI) to a scFv antibody library to systematically inactivate surface proteins expressed by human fibrosarcoma cells followed by a high-throughput assessment of transwell invasion. Results: Using this screen, we have identified CD155 (the poliovirus receptor) as a mediator of tumor cell invasion through its role in migration. Knockdown of CD155 by FALI or by RNAi resulted in a significant decrease in transwell migration of HT1080 fibrosarcoma cells towards a serum chemoattractant. CD155 was found to be highly expressed in multiple cancer cell lines and primary tumors including glioblastoma (GBM). Knockdown of CD155 also decreased migration of U87MG GBM cells. CD155 is recruited to the leading edge of migrating cells where it colocalizes with actin and αv-integrin, known mediators of motility and adhesion. Knockdown of CD155 also altered cellular morphology, resulting in cells that were larger and more elongated than controls when plated on a Matrigel substrate. Conclusion: These results implicate a role for CD155 in mediating tumor cell invasion and migration and suggest that CD155 may contribute to tumorigenesis.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/1471-2407-4-73en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC524493/pdf/en_US
dash.licenseLAA
dc.titleCD155/PVR Plays a Key Role in Cell Motility During Tumor Cell Invasion and Migrationen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBMC Canceren_US
dash.depositing.authorLouis, David Neil
dc.date.available2011-04-26T01:44:00Z
dash.affiliation.otherHMS^Pathologyen_US
dc.identifier.doi10.1186/1471-2407-4-73*
dash.authorsorderedfalse
dash.contributor.affiliatedLouis, David


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