Insulin Signaling Regulates Mitochondrial Function in Pancreatic β-Cells
| Title: | Insulin Signaling Regulates Mitochondrial Function in Pancreatic β-Cells |
| Author: |
Liu, Siming; Okada, Terumasa; Assmann, Anke; Soto, Jamie; Bugger, Heiko; Shirihai, Orian S.; Abel, E. Dale; Liew, Chong-Wee; Kulkarni, Rohit Narayan
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Liu, Siming, Terumasa Okada, Anke Assmann, Jamie Soto, Chong Wee Liew, Heiko Bugger, Orian S. Shirihai, E. Dale Abel, and Rohit N. Kulkarni. 2009. Insulin signaling regulates mitochondrial function in pancreatic β-ccells. PLoS ONE 4(11): e7983. |
| Full Text & Related Files: |
2776992.pdf (1.509Mb; PDF) 
|
| Abstract: | Insulin/IGF-I signaling regulates the metabolism of most mammalian tissues including pancreatic islets. To dissect the mechanisms linking insulin signaling with mitochondrial function, we first identified a mitochondria-tethering complex in β-cells that included glucokinase (GK), and the pro-apoptotic protein, BADS. Mitochondria isolated from β-cells derived from β-cell specific insulin receptor knockout (βIRKO) mice exhibited reduced BADS, GK and protein kinase A in the complex, and attenuated function. Similar alterations were evident in islets from patients with type 2 diabetes. Decreased mitochondrial GK activity in βIRKOs could be explained, in part, by reduced expression and altered phosphorylation of BADS. The elevated phosphorylation of p70S6K and JNK1 was likely due to compensatory increase in IGF-1 receptor expression. Re-expression of insulin receptors in βIRKO cells partially restored the stoichiometry of the complex and mitochondrial function. These data indicate that insulin signaling regulates mitochondrial function and have implications for β-cell dysfunction in type 2 diabetes. |
| Published Version: | doi:10.1371/journal.pone.0007983 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2776992/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4875888 |
| Downloads of this work: |
This item appears in the following Collection(s)
-
HMS Scholarly Articles [16853]
Contact administrator regarding this item (to report mistakes or request changes)
