Show simple item record

dc.contributor.authorWaters, Jennifer Catherine
dc.date.accessioned2011-04-26T02:05:53Z
dc.date.issued2009
dc.identifier.citationWaters, Jennifer C. 2009. Accuracy and precision in quantitative fluorescence microscopy. The Journal of Cell Biology 185(7): 1135-1148.en_US
dc.identifier.issn0021-9525en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4875892
dc.description.abstractThe light microscope has long been used to document the localization of fluorescent molecules in cell biology research. With advances in digital cameras and the discovery and development of genetically encoded fluorophores, there has been a huge increase in the use of fluorescence microscopy to quantify spatial and temporal measurements of fluorescent molecules in biological specimens. Whether simply comparing the relative intensities of two fluorescent specimens, or using advanced techniques like Förster resonance energy transfer (FRET) or fluorescence recovery after photobleaching (FRAP), quantitation of fluorescence requires a thorough understanding of the limitations of and proper use of the different components of the imaging system. Here, I focus on the parameters of digital image acquisition that affect the accuracy and precision of quantitative fluorescence microscopy measurements.en_US
dc.language.isoen_USen_US
dc.publisherThe Rockefeller University Pressen_US
dc.relation.isversionofdoi:10.1083/jcb.200903097en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2712964/pdf/en_US
dash.licenseLAA
dc.titleAccuracy and Precision in Quantitative Fluorescence Microscopyen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalThe Journal of Cell Biologyen_US
dash.depositing.authorWaters, Jennifer Catherine
dc.date.available2011-04-26T02:05:53Z
dash.affiliation.otherHMS^Cell Biologyen_US
dc.identifier.doi10.1083/jcb.200903097*
dash.contributor.affiliatedWaters, Jennifer


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record