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dc.contributor.authorSpringer, Timothy A.
dc.contributor.authorZhu, Jianghai
dc.contributor.authorXiao, Tsan
dc.date.accessioned2011-04-28T04:41:03Z
dc.date.issued2008
dc.identifier.citationSpringer, Timothy A., Jianghai Zhu, and Tsan Xiao. 2008. Structural basis for distinctive recognition of fibrinogen γC peptide by the platelet integrin αIIbβ3. The Journal of Cell Biology 182(4): 791-800.en_US
dc.identifier.issn0021-9525en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4878928
dc.description.abstractHemostasis and thrombosis (blood clotting) involve fibrinogen binding to integrin αIIbβ3 on platelets, resulting in platelet aggregation. αvβ3 binds fibrinogen via an Arg-Asp-Gly (RGD) motif in fibrinogen's α subunit. αIIbβ3 also binds to fibrinogen; however, it does so via an unstructured RGD-lacking C-terminal region of the γ subunit (γC peptide). These distinct modes of fibrinogen binding enable αIIbβ3 and αvβ3 to function cooperatively in hemostasis. In this study, crystal structures reveal the integrin αIIbβ3–γC peptide interface, and, for comparison, integrin αIIbβ3 bound to a lamprey γC primordial RGD motif. Compared with RGD, the GAKQAGDV motif in γC adopts a different backbone configuration and binds over a more extended region. The integrin metal ion–dependent adhesion site (MIDAS) Mg2+ ion binds the γC Asp side chain. The adjacent to MIDAS (ADMIDAS) Ca2+ ion binds the γC C terminus, revealing a contribution for ADMIDAS in ligand binding. Structural data from this natively disordered γC peptide enhances our understanding of the involvement of γC peptide and integrin αIIbβ3 in hemostasis and thrombosis.en_US
dc.language.isoen_USen_US
dc.publisherThe Rockefeller University Pressen_US
dc.relation.isversionofdoi:10.1083/jcb.200801146en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518716/pdf/en_US
dash.licenseLAA
dc.titleStructural basis for distinctive recognition of fibrinogen γC peptide by the platelet integrin αIIbβ3en_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalThe Journal of Cell Biologyen_US
dash.depositing.authorSpringer, Timothy A.
dc.date.available2011-04-28T04:41:03Z
dash.affiliation.otherHMS^Pathologyen_US
dash.affiliation.otherHMS^Pathologyen_US
dc.identifier.doi10.1083/jcb.200801146*
dash.contributor.affiliatedZhu, Jianghai
dash.contributor.affiliatedSpringer, Timothy


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