miRNA Profiling of Naïve, Effector and Memory CD8 T Cells

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miRNA Profiling of Naïve, Effector and Memory CD8 T Cells

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Title: miRNA Profiling of Naïve, Effector and Memory CD8 T Cells
Author: Wu, Haoquan; Neilson, Joel R.; Kumar, Priti; Shankar, Premlata; Manjunath, N.; Manocha, Monika; Sharp, Phillip A.

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Citation: Wu, Haoquan, Joel R. Neilson, Priti Kumar, Monika Manocha, Premlata Shankar, Phillip A. Sharp, and N. Manjunath. 2007. miRNA profiling of naïve, effector and memory CD8 T cells. PLoS ONE 2(10): e1020.
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Abstract: microRNAs have recently emerged as master regulators of gene expression during development and cell differentiation. Although profound changes in gene expression also occur during antigen-induced T cell differentiation, the role of miRNAs in the process is not known. We compared the miRNA expression profiles between antigen-specific naïve, effector and memory CD8+ T cells using 3 different methods-small RNA cloning, miRNA microarray analysis and real-time PCR. Although many miRNAs were expressed in all the T cell subsets, the frequency of 7 miRNAs (miR-16, miR-21, miR-142-3p, miR-142-5p, miR-150, miR-15b and let-7f) alone accounted for ∼60% of all miRNAs, and their expression was several fold higher than the other expressed miRNAs. Global downregulation of miRNAs (including 6/7 dominantly expressed miRNAs) was observed in effector T cells compared to naïve cells and the miRNA expression levels tended to come back up in memory T cells. However, a few miRNAs, notably miR-21 were higher in effector and memory T cells compared to naïve T cells. These results suggest that concomitant with profound changes in gene expression, miRNA profile also changes dynamically during T cell differentiation. Sequence analysis of the cloned mature miRNAs revealed an extensive degree of end polymorphism. While 3′end polymorphisms dominated, heterogeneity at both ends, resembling drosha/dicer processing shift was also seen in miR-142, suggesting a possible novel mechanism to generate new miRNA and/or to diversify miRNA target selection. Overall, our results suggest that dynamic changes in the expression of miRNAs may be important for the regulation of gene expression during antigen-induced T cell differentiation. Our study also suggests possible novel mechanisms for miRNA biogenesis and function.
Published Version: doi:10.1371/journal.pone.0001020
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2000354/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4879183
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