dc.contributor.author | Mkhwanazi, Nompumelelo | |
dc.contributor.author | Thobakgale, Christina F. | |
dc.contributor.author | van der Stok, Mary | |
dc.contributor.author | Reddy, Shabashini | |
dc.contributor.author | Mncube, Zenele | |
dc.contributor.author | Chonco, Fundisiwe | |
dc.contributor.author | Walker, Bruce David | |
dc.contributor.author | Altfeld, Marcus | |
dc.contributor.author | Goulder, Philip J. | |
dc.contributor.author | Ndung'u, Thumbi | |
dc.date.accessioned | 2011-05-10T00:42:53Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Mkhwanazi, Nompumelelo, Christina F. Thobakgale, Mary van der Stok, Shabashini Reddy, Zenele Mncube, Fundisiwe Chonco, Bruce D. Walker, Marcus Altfeld, Philip J. R. Goulder, and Thumbi Ndung'u. 2010. Immunodominant HIV-1-specific HLA-B- and HLA-C-restricted CD8+ T cells do not differ in polyfunctionality. Virology 405(2-3): 483-491. | en_US |
dc.identifier.issn | 0042-6822 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4885960 | |
dc.description.abstract | HIV-1 specific HLA-B-restricted CD8+ T cell responses differ from HLA-C-restricted responses in antiviral effectiveness. To investigate possible reasons for these differences, we characterized the frequency and polyfunctionality of immmunodominant HLA-B*57/B5801- and HLA-Cw*07-restricted CD8+ T cells occurring concurrently in nine study subjects assessing IFN-γ, TNF-α, IL-2, MIP-1β, and CD107a by flow cytometry and analyzed sequence variation in targeted epitopes. HLA-B*57/5801 and HLA-Cw*07 restricted CD8+ T cells did not differ significantly in polyfunctionality (p = 0.84). Possession of three or more functions correlated positively with CD4+ T cell counts (r = 0.85; p = 0.006) and monofunctional CD8+ T cells inversely correlated with CD4 cell counts (r = −0.79; p = 0.05). There were no differences in polyfunctionality of CD8+ T cells specific to wildtype versus mutated epitopes. These results suggest that loss of polyfunctionality and increase in monofunctional HIV-1-specific CD8+ T cells are associated with disease progression independent of restricting HLA allele. Furthermore, sequence variation does not appear to significantly impact CD8+ T cell polyfunctionality in chronic HIV-1 infection. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Academic Press | en_US |
dc.relation.isversionof | doi:10.1016/j.virol.2010.06.002 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954365/pdf/ | en_US |
dash.license | LAA | |
dc.subject | HLA-B*57/5801 | en_US |
dc.subject | HLA-C | en_US |
dc.subject | HIV-1 chronic infection | en_US |
dc.subject | CD8+ T cells | en_US |
dc.subject | polyfunctionality | en_US |
dc.title | Immunodominant HIV-1-specific HLA-B- and HLA-C-restricted CD8+ T cells do not differ in polyfunctionality | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | Virology | en_US |
dash.depositing.author | Walker, Bruce David | |
dc.date.available | 2011-05-10T00:42:53Z | |
dash.affiliation.other | HMS^Medicine-Massachusetts General Hospital | en_US |
dash.affiliation.other | SPH^Immunology and Infectious Diseases | en_US |
dash.affiliation.other | HMS^Medicine-Massachusetts General Hospital | en_US |
dc.identifier.doi | 10.1016/j.virol.2010.06.002 | * |
dash.contributor.affiliated | Goulder, Philip J. | |
dash.contributor.affiliated | Altfeld, Marcus | |
dash.contributor.affiliated | Walker, Bruce | |
dc.identifier.orcid | 0000-0001-6122-9245 | |