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dc.contributor.authorBrodsky, Alexander S
dc.contributor.authorMeyer, Clifford
dc.contributor.authorSwinburne, Ian A
dc.contributor.authorHall, Giles
dc.contributor.authorKeenan, Benjamin J
dc.contributor.authorLiu, Xiaole Shirley
dc.contributor.authorFox, Edward Alvin
dc.contributor.authorSilver, Pamela A.
dc.date.accessioned2011-05-11T02:04:04Z
dc.date.issued2005
dc.identifier.citationBrodsky, Alexander S., Clifford A. Meyer, Ian A. Swinburne, Giles Hall, Benjamin J. Keenan, Xiaole S. Liu, Edward A. Fox, and Pamela A. Silver. 2005. Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells. Genome Biology 6(8): R64.en_US
dc.identifier.issn1465-6906en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4887112
dc.description.abstractBackground: Transcription by RNA polymerase II is regulated at many steps including initiation, promoter release, elongation and termination. Accumulation of RNA polymerase II at particular locations across genes can be indicative of sites of regulation. RNA polymerase II is thought to accumulate at the promoter and at sites of co-transcriptional alternative splicing where the rate of RNA synthesis slows. Results: To further understand transcriptional regulation at a global level, we determined the distribution of RNA polymerase II within regions of the human genome designated by the ENCODE project. Hypophosphorylated RNA polymerase II localizes almost exclusively to 5' ends of genes. On the other hand, localization of total RNA polymerase II reveals a variety of distinct landscapes across many genes with 74% of the observed enriched locations at exons. RNA polymerase II accumulates at many annotated constitutively spliced exons, but is biased for alternatively spliced exons. Finally, RNA polymerase II is also observed at locations not in gene regions. Conclusion: Localizing RNA polymerase II across many millions of base pairs in the human genome identifies novel sites of transcription and provides insights into the regulation of transcription elongation. These data indicate that RNA polymerase II accumulates most often at exons during transcription. Thus, a major factor of transcription elongation control in mammalian cells is the coordination of transcription and pre-mRNA processing to define exons.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/gb-2005-6-8-r64en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1273631/pdf/en_US
dash.licenseLAA
dc.titleGenomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cellsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalGenome Biologyen_US
dash.depositing.authorMeyer, Clifford
dc.date.available2011-05-11T02:04:04Z
dash.affiliation.otherSPH^Biostatisticsen_US
dash.affiliation.otherHMS^Systems Biologyen_US
dash.affiliation.otherSPH^Biostatisticsen_US
dash.affiliation.otherHMS^Medicine-Brigham and Women's Hospitalen_US
dash.affiliation.otherHMS^Systems Biologyen_US
dc.identifier.doi10.1186/gb-2005-6-8-r64*
dash.contributor.affiliatedSilver, Pamela
dash.contributor.affiliatedSwinburne, Ian
dash.contributor.affiliatedMeyer, Clifford
dash.contributor.affiliatedLiu, Xiaole
dash.contributor.affiliatedFox, Edward Alvin


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