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dc.contributor.authorHett, Erik Christian
dc.contributor.authorChao, Michael
dc.contributor.authorRubin, Eric Joseph
dc.date.accessioned2011-05-19T01:34:35Z
dc.date.issued2010
dc.identifier.citationHett, Erik C., Michael C. Chao, and Eric J. Rubin. 2010. Interaction and Modulation of Two Antagonistic Cell Wall Enzymes of Mycobacteria. PLoS Pathogens 6(7): e1001020.en_US
dc.identifier.issn1553-7366en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4891676
dc.description.abstractBacterial cell growth and division require coordinated cell wall hydrolysis and synthesis, allowing for the removal and expansion of cell wall material. Without proper coordination, unchecked hydrolysis can result in cell lysis. How these opposing activities are simultaneously regulated is poorly understood. In Mycobacterium tuberculosis, the resuscitation-promoting factor B (RpfB), a lytic transglycosylase, interacts and synergizes with Rpf-interacting protein A (RipA), an endopeptidase, to hydrolyze peptidoglycan. However, it remains unclear what governs this synergy and how it is coordinated with cell wall synthesis. Here we identify the bifunctional peptidoglycan-synthesizing enzyme, penicillin binding protein 1 (PBP1), as a RipA-interacting protein. PBP1, like RipA, localizes both at the poles and septa of dividing cells. Depletion of the ponA1 gene, encoding PBP1 in M. smegmatis, results in a severe growth defect and abnormally shaped cells, indicating that PBP1 is necessary for viability and cell wall stability. Finally, PBP1 inhibits the synergistic hydrolysis of peptidoglycan by the RipA-RpfB complex in vitro. These data reveal a post-translational mechanism for regulating cell wall hydrolysis and synthesis through protein–protein interactions between enzymes with antagonistic functions.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.ppat.1001020en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912383/pdf/en_US
dash.licenseLAA
dc.subjectcell biologyen_US
dc.subjectmicrobial growth and developmenten_US
dc.subjectmicrobial physiology and metabolismen_US
dc.subjectinfectious diseasesen_US
dc.subjectbacterial infectionsen_US
dc.subjectpublic health and epidemiologyen_US
dc.titleInteraction and Modulation of Two Antagonistic Cell Wall Enzymes of Mycobacteriaen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS Pathogensen_US
dash.depositing.authorHett, Erik Christian
dc.date.available2011-05-19T01:34:35Z
dash.affiliation.otherHMS^Microbiology and Molecular Geneticsen_US
dash.affiliation.otherSPH^Student Stipends^IIDen_US
dash.affiliation.otherSPH^Student Stipendsen_US
dash.affiliation.otherHMS^Stipendees - Div of Medical Sciencesen_US
dash.affiliation.otherHMS^Medicine-Brigham and Women's Hospitalen_US
dash.affiliation.otherSPH^Immunology and Infectious Diseases TBen_US
dc.identifier.doi10.1371/journal.ppat.1001020*
dash.contributor.affiliatedChao, Michael C.
dash.contributor.affiliatedHett, Erik Christian
dash.contributor.affiliatedRubin, Eric


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