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dc.contributor.authorMcKee, Adrienne E.
dc.contributor.authorMinet, Emmanuel
dc.contributor.authorStern, Charlene
dc.contributor.authorRiahi, Shervin
dc.contributor.authorStiles, Charles Dean
dc.contributor.authorSilver, Pamela A.
dc.date.accessioned2011-06-08T17:55:05Z
dc.date.issued2005
dc.identifier.citationMcKee, Adrienne E., Emmanuel Minet, Charlene Stern, Shervin Riahi, Charles D. Stiles, and Pamela A. Silver. 2005. A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain. BMC Developmental Biology 5: 14.en_US
dc.identifier.issn1471-213Xen_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4907903
dc.description.abstractBackground: In eukaryotic cells, RNA-binding proteins (RBPs) contribute to gene expression by regulating the form, abundance, and stability of both coding and non-coding RNA. In the vertebrate brain, RBPs account for many distinctive features of RNA processing such as activity-dependent transcript localization and localized protein synthesis. Several RBPs with activities that are important for the proper function of adult brain have been identified, but how many RBPs exist and where these genes are expressed in the developing brain is uncharacterized. Results: Here we describe a comprehensive catalogue of the unique RBPs encoded in the mouse genome and provide an online database of RBP expression in developing brain. We identified 380 putative RBPs in the mouse genome. Using in situ hybridization, we visualized the expression of 323 of these RBP genes in the brains of developing mice at embryonic day 13.5, when critical fate choice decisions are made and at P0, when major structural components of the adult brain are apparent. We demonstrate i) that 16 of the 323 RBPs examined show neural-specific expression at the stages we examined, and ii) that a far larger subset (221) shows regionally restricted expression in the brain. Of the regionally restricted RBPs, we describe one group that is preferentially expressed in the E13.5 ventricular areas and a second group that shows spatially restricted expression in postmitotic regions of the embryonic brain. Additionally, we find a subset of RBPs that share the same complex pattern of expression, in proliferating regions of the embryonic and postnatal NS and peripheral tissues. Conclusion: Our data show that, in contrast to their proposed ubiquitous involvement in gene regulation, most RBPs are not uniformly expressed. Here we demonstrate the region-specific expression of RBPs in proliferating vs. post-mitotic brain regions as well as cell-type- pecific RBP expression. We identify uncharacterized RBPs that exhibit neural-specific expression as well as novel RBPs that show expression in non-neural tissues. The data presented here and in an online database provide a visual filter for the functional analysis of individual RBPs.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi://10.1186/1471-213X-5-14en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1199591/pdf/en_US
dash.licenseLAA
dc.titleA Genome-Wide In Situ Hybridization Map of RNA-Binding Proteins reveals Anatomically Restricted Expression in the Developing Mouse Brainen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBMC Developmental Biologyen_US
dash.depositing.authorStiles, Charles Dean
dc.date.available2011-06-08T17:55:05Z
dash.affiliation.otherHMS^Microbiology and Molecular Geneticsen_US
dash.affiliation.otherHMS^Systems Biologyen_US
dc.identifier.doi10.1186/1471-213X-5-14*
dash.contributor.affiliatedSilver, Pamela
dash.contributor.affiliatedStiles, Charles


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