DNA Index Determination with Automated Cellular Imaging System (ACIS) in Barrett's Esophagus: Comparison with CAS 200

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DNA Index Determination with Automated Cellular Imaging System (ACIS) in Barrett's Esophagus: Comparison with CAS 200

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Title: DNA Index Determination with Automated Cellular Imaging System (ACIS) in Barrett's Esophagus: Comparison with CAS 200
Author: Huang, Qin; Yu, Chenggong; Klein, Michael; Fang, James; Goyal, Raj K.

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Citation: Huang, Qin, Chenggong Yu, Michael Klein, James Fang, and Raj K. Goyal. 2005. DNA index determination with Automated Cellular Imaging System (ACIS) in Barrett's esophagus: Comparison with CAS 200. BMC Clinical Pathology 5:7.
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Abstract: Background: For solid tumors, image cytometry has been shown to be more sensitive for diagnosing DNA content abnormalities (aneuploidy) than flow cytometry. Image cytometry has often been performed using the semi-automated CAS 200 system. Recently, an Automated Cellular Imaging System (ACIS) was introduced to determine DNA content (DNA index), but it has not been validated. Methods: Using the CAS 200 system and ACIS, we compared the DNA index (DI) obtained from the same archived formalin-fixed and paraffin embedded tissue samples from Barrett's esophagus related lesions, including samples with specialized intestinal metaplasia without dysplasia, low-grade dysplasia, high-grade dysplasia and adenocarcinoma. Results: Although there was a very good correlation between the DI values determined by ACIS and CAS 200, the former was 25% more sensitive in detecting aneuploidy. ACIS yielded a mean DI value 18% higher than that obtained by CAS 200 (p < 0.001; paired t test). In addition, the average time required to perform a DNA ploidy analysis was shorter with the ACIS (30–40 min) than with the CAS 200 (40–70 min). Results obtained by ACIS gave excellent inter-and intra-observer variability (coefficient of correlation >0.9 for both, p < 0.0001). Conclusion: Compared with the CAS 200, the ACIS is a more sensitive and less time consuming technique for determining DNA ploidy. Results obtained by ACIS are also highly reproducible.
Published Version: doi:10.1186/1472-6890-5-7
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1201134/pdf/
http://www.biomedcentral.com/1472-6890/5/7
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4910918
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