Browsing FAS Theses and Dissertations by Keyword "zinc finger"
Now showing items 1-3 of 3
-
Improving Zinc Finger Nucleases - Strategies for Increasing Gene Editing Activities and Evaluating Off-Target Effects
(2012-08-23)Zinc finger nucleases (ZFNs) induce double-strand DNA breaks at specific recognition sites. ZFNs can dramatically increase the efficiency of incorporating desired insertions, deletions, or substitutions in living cells. ... -
A mutational analysis of the Bacillus subtilis competence helicase ComFA
(2014-06-06)Genetic competence is a developmental process in bacteria that allows natural transformation. Competent Gram positive bacteria such as Bacillus subtilis carry a cytosolic helicase which is required for efficient transformation. ... -
Synthetic Biology Approaches to Engineering Human Cells
(2013-08-21)The field of synthetic biology seeks to revolutionize the scope and scale of what is currently feasible by genetic engineering. By focusing on engineering general signal processing platforms using modular genetic parts and ...