Now showing items 1-10 of 35
Regulation of Stem Cell Metabolism by the Lin28/let-7 Axis
My PhD thesis is focused on two fundamental aspects of stem cell metabolism: (1) the role of Lin28 in programming stem cell metabolism, and (2) how metabolism in turn fuels and governs pluripotency. Our studies led us to ...
Non-Enzymatic Copying of Nucleic Acid Templates
All known living cells contain a complex set of molecular machinery to support their growth and replication. However, the earliest cells must have been much simpler, consisting of a compartment and a genetic material to ...
Cellular and Systemic Metabolic Adaptations to Energy Status
My thesis work has focused on physiological adaptations to nutrient stress; from genes, environment and hormonal perspective, and how failure of these systems result in common chronic diseases.
Profiling and Improving the Specificity of Site-Specific Nucleases
Programmable site-specific endonucleases are useful tools for genome editing and may lead to novel therapeutics to treat genetic diseases. TALENs can be designed to cleave chosen DNA sequences. To better understand TALEN ...
Oxidative Assembly of the Outer Membrane Lipopolysaccharide Translocon LptD/E and Progress towards Its X-Ray Crystal Structure
Lipopolysaccharide (LPS) is the glycolipid that comprises the outer leaflet of the Gram-negative outer membrane (OM). Because it is essential in nearly all Gram-negative species, and because it is responsible for making ...
Modulators of Cellular and Biochemical PRC2 Activity
EZH2 is a SET domain-containing methyltransferase and the catalytic component of the multimeric Polycomb- group (PcG) protein complex, PRC2. When in complex with other PRC2 members (EED, SUZ12, AEBP2, and RBBP4), EZH2 ...
Characterization of Protein-Metabolite and Protein-Substrate Interactions of Disease Genes
Discovery of protein-metabolite and protein-substrate interactions that can specifically regulate genes involved in human biology is an important pursuit, as the study of such interactions can expand our understanding of ...
Genome-wide Analysis of Ctcf-RNA Interactions
Ctcf is a "master regulator" of the genome that plays a role in a variety of gene regulatory functions as well as in genome architecture. Evidence from studying the epigenetic process of X-chromosome inactivation suggests ...
From DNA sequence recognition to directional chromosome segregation: Information transfer in the translocase protein SpoIIIE
Faithful chromosome segregation is essential for all living organisms. Bacterial chromosome segregation utilizes highly conserved directional SpoIIIE/FtsK translocases to move large DNA molecules between spatially separated ...
Defining and Targeting Transcriptional Pathways in Leukemia Stem Cells
Acute myeloid leukemia (AML) is a clonal neoplastic disorder organized as a cellular hierarchy, with the self-renewing leukemia stem cell (LSC) at the apex. Recurrent mutations in transcription factors (TF) and epigenetic ...