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Envelope biogenesis and spore formation in Bacillus subtilis
(2016-05-02)
The bacterial cell wall exoskeleton (or peptidoglycan) protects cells from osmotic lysis, specifies their shape, and its biosynthetic pathway is a widely-exploited antibiotic target. My thesis research focused on defining ...
Investigations of the Biosynthesis and Structure of Colibactin, a Cytotoxin Made by Human-Associated Escherichia Coli
(2016-01-13)
Humans exist in symbiosis with trillions of bacteria that are collectively referred to as the human microbiota. While commensal microbes are essential for health, some resident microbes can promote disease. Certain strains ...
DNA Polymerase Exchange and Lesion Bypass in Escherichia Coli
(2015-11-13)
Translesion synthesis (TLS) alleviates replication stalling at DNA lesions. Bypass of lesions by specialized translesion DNA polymerases involves exchange with high-fidelity replicative polymerases. As a consequence of ...
Identifying targets for potentiators in S. aureus using chemical genetic approaches
(2016-09-29)
Staphylococcus aureus is a highly feared Gram-positive pathogen. The rise in antibiotic resistance has made S. aureus infections intractable. To find new ways to treat S. aureus infections, it is important to understand ...
Clp-Mediated Regulation of the Mycobacterial Cell Cycle
(2017-04-12)
Tuberculosis is currently the most deadly infectious disease worldwide. Given the high rates of treatment failure, new antibiotics are desperately needed. Antibacterial drugs typically target DNA replication, cell growth, ...
Biophysical Studies of the Lpt Pathway
(2017-07-06)
The outer membrane (OM) of Gram-negative bacteria is impermeable to many antibiotics because its outer leaflet is composed entirely of lipopolysaccharide (LPS), a large glycolipid with an extracellular saccharide that is ...
Phage Assisted Evolutions for Engineering Biosynthetic Pathways
(2018-05-14)
Phage Assisted Continuous Evolution (PACE) and its derivative techniques are powerful tools for evolving improved and altered enzymatic activities. Prior to this work, no one had demonstrated the applicability of these ...