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Postnatal Genome Editing With CRISPR
(2016-05-18)
Targeted genome editing holds tremendous promise for permanent correction of many genetic diseases. The recently developed CRISPR/Cas9 genome-editing tool exhibits facile programmability and robust gene-editing efficiency, ...
Investigating Mechanisms of DNA Double Strand Break Joining of Switch Regions During IgH Class Switch Recombination
(2016-05-19)
During B cell development, RAG endonuclease cleaves immunoglobulin heavy chain (IgH) V, D, and J gene segments and orchestrates their fusion as deletional events that assemble a V(D)J exon in the same transcriptional ...
Isogenic Human Pluripotent Stem Cell Models of Cardiovascular Disease-Associated Genetic Variation
(2016-03-30)
A complex interplay of genetic and environmental factors underlies the development of common human diseases such as cardiovascular disease. Despite widespread use of existing medications, coronary heart disease (CHD), ...
Adaptive Evolution of the SIV Envelope Protein During Early SIV Infection
(2016-03-08)
Primate lentiviruses (PLVs), including human immunodeficiency virus type 1 (HIV-1), HIV type 2 (HIV-2), and the simian immunodeficiency viruses (SIVs), cause persistent lifelong infections despite the presence of virus-specific ...
Elucidation of Mechanisms of Fetal Hemoglobin Regulation by CRISPR/Cas9 Mediated Genome Editing
(2016-03-14)
Despite nearly complete understanding of the genetics of the β-hemoglobinopathies for several decades, definitive treatment options have lagged behind. Fetal hemoglobin (HbF) reinduction represents a “silver bullet” for ...
Mechanisms Controlling the Cell Envelope Remodeling Activities of the Escherichia Coli Cytokinetic Ring
(2016-05-13)
In Escherichia coli, cytokinesis requires the constriction of all the cell envelope layers: the inner membrane, the peptidoglycan (PG) cell wall and the outer membrane (OM). Such dramatic cell envelope remodeling requires ...
Diverse Gene Regulatory Mechanisms Pattern the Vertebrate Embryo
(2016-05-19)
Development is the elegant and complex process by which a single celled embryo grows into a fully formed and functional adult. One of the major challenges in the process of development is that although each cell in an ...
Characterization of Dopamine-Responsive Serotonergic Neurons Underlying Aggression Modulation
(2016-04-04)
The monoaminergic neuromodulatory systems (including the noraderenergic, serotonergic, and dopaminergic neuronal systems) have long been thought of as the custodians of mental health. Dysfunction amongst these neural ...
Functional characterization of genetic alterations in cancer
(2016-05-18)
The comprehensive identification of genetic alterations is critical to understanding the pathophysiology of cancer. Recent advances in sequencing technology have enabled the detailed description of cancer genomes. However, ...
Regulation of Behavioral Arousal and Quiescence in C. elegans
(2016-05-13)
Animals switch between periods of behavioral quiescence and arousal in response to environmental, circadian, or developmental cues. C. elegans exhibit periods of behavioral quiescence during larval molts (termed lethargus) ...