Now showing items 11-16 of 16
When the Circadian Clock Cannot Be Set: An Unstable Singularity Underlies Stochastic Phasing of the Circadian Clock in Individual Cyanobacterial Cells
The endogenous circadian clock synchronizes with environmental time by appropriately resetting its phase in response to external cues. Of note, some resetting stimuli induce attenuated oscillations of clock output, which ...
Programming molecular behavior: development and applications of autonomous DNA synthesis cascades
DNA serves many functional roles in living organisms, and dynamic DNA nanotechnology seeks to expand this set of DNA-based behaviors through the rational design of new functions. In the past 20 years, the predictable and ...
Characterization and applications of novel DNA- and RNA-targeting CRISPR effectors
The development of molecular tools from natural discovery has played an integral role in the history of modern biology. Genome editing systems derived from the CRISPR bacterial immune system have enabled substantial increases ...
Genomic Analysis of Viral Outbreaks
Due to recent advances in sequencing technologies, genomics has emerged as a powerful tool for combating viral outbreaks. In this dissertation, I show how genomic data can be used to understand the transmission, as well ...
Evolutionary dynamics of CRISPR gene drives
The alteration of wild populations has been discussed as a solution to a number of humanity’s most pressing ecological and public health problems. Enabled by the recent revolution in genome editing, CRISPR gene drive ...
The Thermodynamics of Carbon Redox Biochemistry: A Quantum Chemical Approach
Thermodynamics plays an increasingly important role in modeling and engineering metabolism. Here we developed quantum chemistry approaches to predicting the thermodynamics of biochemical reactions in order to help fill in ...