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Now showing items 11-20 of 127
Oligopaints: Highly Programmable Oligonucleotide Probes for Visualizing Genomes in Situ
(2015-01-29)
Fluorescence in situ hybridization (FISH) is a powerful assay that can visualize the position of DNA and RNA molecules in individual cells. Here, I describe the development of a method that utilizes complex oligonucleotide ...
Bioinformatic and molecular study of the regulation of SIRT3 expression
(2015-01-29)
Calorie restriction (CR) is a dietary intervention that extends lifespan, delays the onset of age-related diseases, and induces a wide-ranging metabolic adaptation in multiple model organisms. One of its primary effectors ...
Mechanisms of Stem Cell Maintenance and Cell Differentiation in the Intestinal Epithelium
(2015-01-23)
Constant regeneration of the intestinal epithelium, a dynamic tissue with vital digestive and barrier functions, depends on proliferation of resident stem cells and their differentiation into mature cell types. This ...
The Mechanistic Basis of Dynein Microtubule Binding and Its Regulation
(2015-01-22)
Eukaryotic cells use a diverse toolbox of cytoskeletal motors to transport and position cellular materials in space and time. Two microtubule-based motors—kinesin and dynein—transport organelles, RNA and protein cargos ...
Spatial Organizing Principles of Cytokinesis Signaling
(2015-09-23)
Cytokinesis is the final step of cell division, in which the cytoplasm of one cell is physically divided into two. Animal cells integrate multiple positional cues from the mitotic spindle to decide where to position their ...
Defining the Essential Function of Yeast Hsf1 Reveals a Compact Transcriptional Program for Maintaining Eukaryotic Proteostasis
(2016-05-14)
Despite its eponymous association with proteotoxic stress, heat shock factor 1 (Hsf1 in yeast and HSF1 in mammals) is required for viability of yeast and many human cancer cells, yet its essential role remains undefined. ...
Combinatorial Pathway Modulation Toward Ex Vivo Maintenance and Propagation of Hematopoietic Stem Cells
(2016-05-18)
Hematopoietic stem cells (HSCs) sustain continuous turnover and maintenance of all blood lineages through organismal lifespan. The extensive regenerative potential of HSCs has been harnessed in transplantation medicine ...
Transcriptional Control of Maternal-Fetal Immune Tolerance
(2016-05-18)
Human leukocyte antigens (HLA) are important determinants of self-nonself immune recognition. HLA-G, uniquely expressed in the placenta, is believed to be key to fetus-induced immune tolerance during pregnancy. The ...
Resistance to Targeted Therapy in Sonic Hedgehog Subgroup Medulloblastoma: Mechanisms and Treatment Strategies
(2016-05-17)
Aberrant activation of the Sonic Hedgehog (Shh) signaling network is implicated in many human cancers, including the most common cancer, basal cell carcinoma (BCC) and the brain tumor medulloblastoma. Suppressing Shh ...
Investigating the Role of Sox2 in Stomach Tissue Homeostasis and Cancer.
(2015-05-16)
The transcription factor Sox2 is essential for the establishment and maintenance of multiple stem cell populations and its coding region is amplified in certain carcinomas. However, Sox2’s role in stomach homeostasis and ...