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Isogenic Human Pluripotent Stem Cell Models of Cardiovascular Disease-Associated Genetic Variation
(2016-03-30)
A complex interplay of genetic and environmental factors underlies the development of common human diseases such as cardiovascular disease. Despite widespread use of existing medications, coronary heart disease (CHD), ...
Adaptive Evolution of the SIV Envelope Protein During Early SIV Infection
(2016-03-08)
Primate lentiviruses (PLVs), including human immunodeficiency virus type 1 (HIV-1), HIV type 2 (HIV-2), and the simian immunodeficiency viruses (SIVs), cause persistent lifelong infections despite the presence of virus-specific ...
Elucidation of Mechanisms of Fetal Hemoglobin Regulation by CRISPR/Cas9 Mediated Genome Editing
(2016-03-14)
Despite nearly complete understanding of the genetics of the β-hemoglobinopathies for several decades, definitive treatment options have lagged behind. Fetal hemoglobin (HbF) reinduction represents a “silver bullet” for ...
Mechanisms Controlling the Cell Envelope Remodeling Activities of the Escherichia Coli Cytokinetic Ring
(2016-05-13)
In Escherichia coli, cytokinesis requires the constriction of all the cell envelope layers: the inner membrane, the peptidoglycan (PG) cell wall and the outer membrane (OM). Such dramatic cell envelope remodeling requires ...
Regulation of Behavioral Arousal and Quiescence in C. elegans
(2016-05-13)
Animals switch between periods of behavioral quiescence and arousal in response to environmental, circadian, or developmental cues. C. elegans exhibit periods of behavioral quiescence during larval molts (termed lethargus) ...
Strategies for Studying Chromatin Regulation and Organization
(2016-04-22)
The epigenetic regulation of gene transcription underlies the diversity between species and within organisms. Novel implementation of existing methods and the development of original technologies continue to contribute ...
Mutations in the Charged Domain of CBX2 Disrupt PRC1 Function in Vivo
(2016-05-06)
Epigenetics inheritance is a phenomenon where a cell state is inherited across cellular divisions. It is important for maintaining differentiated cells types, and thus is fundamental to the development of multicellular ...
Transcriptional Regulation of Nodal Target Genes in Early Zebrafish Development
(2016-05-06)
Nodal signaling is one of the principal players in the process of gastrulation, during which the primary germ layers (endoderm, mesoderm, and ectoderm) are formed and organized in their proper locations. During my PhD I ...
Towards Sense Codon Reassignment in Escherichia Coli
(2015-10-23)
Expansion of the genetic code through engineering of the translation machinery has vastly increased the chemical repertoire of the proteome. Incorporation of non-canonical amino acids (ncAAs) entails use of engineered ...
A Gain of Function Senescence Bypass Screen Identifies the Homeobox Transcription Factor DLX2 as a Regulator of ATM-P53 Signaling
(2016-01-26)
Senescence stimuli activate multiple tumor suppressor pathways to initiate cycle arrest and a differentiation program characteristic of senescent cells. We performed a two-stage, gain-of function screen to select for the ...