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Non-Enzymatic Copying of Nucleic Acid Templates
(2014-02-25)
All known living cells contain a complex set of molecular machinery to support their growth and replication. However, the earliest cells must have been much simpler, consisting of a compartment and a genetic material to ...
Investigating Cytoskeletal Motor Mechanisms using DNA Nanotechnology
(2014-02-25)
The microtubule cytoskeleton plays a vital role in the spatial-temporal organization of subcellular cargo required to maintain homeostasis and direct cell division. Cytoplasmic dynein and kinesin are opposite-polarity, ...
Trapping and Manipulating Single Molecules of DNA
(2014-02-25)
This thesis presents the development and application of nanoscale techniques to trap and
Linking Human Genetic Variation to Mitochondrial Metabolism
(2014-06-06)
Genetic variation has a powerful impact on human metabolism and disease. Traditionally, this relationship has either been studied at a high level using top-down descriptive studies of patients with genetically defined ...
The Role of HEXIM1 in the Transcriptional Regulation of Neural Crest Differentiation and Melanoma
(2014-06-06)
Recent evidence suggests that leflunomide, a dihydroorotate dehydrogenase (DHODH) inhibitor, disrupts neural crest development and melanoma pathogenesis via inhibiting transcription elongation. DHODH is an enzyme in the ...
Towards construction of synthetic ribosomes and a self-replicating system
(2014-06-06)
In 2006, the Church Group, using biochemical approaches, hypothesized that ∼ 151 biomolecular components from Escherichia coli and its bacteriophages may be sufficient to enable rapid and accurate self–replication ...
Small-molecule probes to explore cancer
(2014-06-06)
Small molecules play important roles in therapeutics and drug discovery. Significant progress has been made by the chemical biology community to discover small-molecule probes to explore biological processes and to treat ...
Chemical Probes and the Exploration of Bromodomains in Cancer Biology
(2014-06-06)
The post-translational modification of histones and their interaction with transcription factors is essential to gene regulation. Furthermore, these targets would greatly benefit from probe molecules to fully elucidate ...
Genome-wide Analysis of Ctcf-RNA Interactions
(2014-06-06)
Ctcf is a "master regulator" of the genome that plays a role in a variety of gene regulatory functions as well as in genome architecture. Evidence from studying the epigenetic process of X-chromosome inactivation suggests ...
Epigenetic shielding: 5-hydroxymethylcytosine and 5-carboxylcytosine modulate UV induction of DNA photoproducts
(2014-06-06)
Maintaining the balance between dynamic DNA methylation and demethylation is crucial to mammalian development and pathogenesis. In vitro methylation at the C-5 position of cytosine enhances cyclobutane pyrimidine dimer ...