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Trapping and Manipulating Single Molecules of DNA
(2014-02-25)
This thesis presents the development and application of nanoscale techniques to trap and
Small-molecule probes to explore cancer
(2014-06-06)
Small molecules play important roles in therapeutics and drug discovery. Significant progress has been made by the chemical biology community to discover small-molecule probes to explore biological processes and to treat ...
Identification and Characterization of Intermediates during Folding on the β-Barrel Assembly Machine in Escherichia coli
(2014-06-06)
β-barrel membrane proteins play important structural and functional roles in Gram negative bacteria and in mitochondria and chloroplasts of eukaryotes. A conserved machine is responsible for the folding and insertion of ...
Targeting `Undruggable' Cancer Proteins with Irreversible Small Molecule Inhibitors: Her3 and KRas
(2014-06-06)
With the lighting speed revolution of technologies in chemistry and biology, increasing number of proteins which eluded scientists' efforts to block them before and were labeled as `undruggable', were successfully targeted ...
Study on Bacterial Protein Synthesis System toward the Incorporation of D-Amino Acid & Synthesis of 2'-deoxy-3'-mercapto-tRNA
(2014-06-06)
Life is anti-entropic and highly organized phenomenon with two characteristics reinforcing each other: homochirality and the stereospecific catalysis of chemical reactions. The exclusive presence of L-amino acids and ...
Discovery and Characterization of Novel Bioactive Peptides and a Natural ERRalpha Ligand
(2013-10-17)
Metabolites and peptides have a central role in biology that is often overlooked. Despite the importance of metabolites in key protein-metabolite interactions (PMIs), the extent and identity of these interactions is not ...
Discovery of bioactive lipids and lipid pathways in cell death and disease
(2014-06-06)
Apoptosis is an intricately regulated cellular process required for the health and homeostasis of living systems. The mitochondrial apoptotic pathway depends on the BCL-2 family of pro- and anti-apoptotic members whose ...
Directed Evolution of Sortase Activity and Specificity
(2014-06-06)
Nature employs complex networks of protein-tailoring enzymes to effect the post-translational modification of proteins in vivo. By comparison, modern chemical methods rely upon either nonspecific labeling techniques or ...
Small-Molecule Modulators of Pancreatic Ductal Cells: Histone Methyltransferases and \(\beta\)-Cell Transdifferentiation
(2013-03-06)
Small molecules are important not only for treating human diseases but also for studying disease-related biological processes. This dissertation focuses on the effects of small molecules on pancreatic ductal adenocarcinoma ...
The Biochemistry and Physiology of Peptidases
(2013-03-14)
Peptidases regulate important physiological processes by controlling levels of bioactive peptides and occasionally through noncatalytic processes. This thesis presents a study of prolyl endopeptidase-like (PREPL), which ...