The Role Played by Cell-Substrate Interactions in the Pathogenesis of Osteoclast-Mediated Peri-Implant Osteolysis

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The Role Played by Cell-Substrate Interactions in the Pathogenesis of Osteoclast-Mediated Peri-Implant Osteolysis

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dc.contributor.author Crotti, Tania N
dc.contributor.author Matsuzaki, Kenichiro
dc.contributor.author Gravallese, Ellen M
dc.contributor.author Goldring, Steven R
dc.contributor.author Shen, Zhenxin
dc.contributor.author McHugh, Kevin P.
dc.contributor.author Bierbaum, Benjamin E.
dc.date.accessioned 2011-07-07T18:37:24Z
dc.date.issued 2006
dc.identifier.citation Shen, Zhenxin, Tania N. Crotti, Kevin P. McHugh, Kenichiro Matsuzaki, Ellen M. Gravallese, Benjamin E. Bierbaum, and Steven R. Goldring. 2006. The role played by cell-substrate interactions in the pathogenesis of osteoclast-mediated peri-implant osteolysis. Arthritis Research & Therapy 8(3): R70. en_US
dc.identifier.issn 1478-6354 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:5011925
dc.description.abstract Prosthetic wear debris-induced peri-implant osteolysis is a major cause of aseptic loosening after total joint replacement. In this condition, wear particles released from the implant components induce a granulomatous inflammatory reaction at the interface between implant and adjacent bone, leading to progressive bone resorption and loss of fixation. The present study was undertaken to characterize definitively the phenotype of osteoclast-like cells associated with regions of peri-implant focal bone resorption and to compare the phenotypic features of these cells with those of mononucleated and multinucleated cells associated with polyethylene wear particles. Peri-implant tissues were obtained from patients undergoing hip revision surgery for aseptic loosening after total joint replacement. Cells were examined for the expression of several markers associated with the osteoclast phenotype using immunohistochemistry, histochemistry, and/or in situ hybridization. CD68 protein, a marker expressed by multiple macrophage lineage cell types, was detected in mononucleated and multinucleated cells associated with polyethylene particles and the bone surface. Cathepsin K and tartrate-resistant acid phosphatase were expressed highly in both mononucleated and multinucleated cells associated with the bone surface. Levels of expression were much lower in cells associated with polyethylene particles. High levels of β3 integrin protein were detected in cells in contact with bone. Multinucleated cells associated with polyethylene particles exhibited faint positive staining. Calcitonin receptor mRNA expression was detected solely in multinucleated cells present in resorption lacunae on the bone surface and was absent in cells associated with polyethylene particles. Our findings provide further evidence that cells expressing the full repertoire of osteoclast phenotypic markers are involved in the pathogenesis of peri-implant osteolysis after total joint replacement. They also demonstrate that foreign body giant cells, although believed to be phenotypically and functionally distinct from osteoclasts, express many osteoclast-associated genes and gene products. However, the levels and patterns of expression of these genes in the two cell types differ. We speculate that, in addition to the role of cytokines and growth factors, the substrate with which these cells interact plays a critical role in their differential phenotypic and functional properties. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/ar1938 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1526628/pdf/ en_US
dash.license LAA
dc.title The Role Played by Cell-Substrate Interactions in the Pathogenesis of Osteoclast-Mediated Peri-Implant Osteolysis en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Arthritis Research & Therapy en_US
dash.depositing.author Shen, Zhenxin
dc.date.available 2011-07-07T18:37:24Z
dash.affiliation.other HMS^Orthopedic Surgery- Beth Israel-Deaconess en_US
dash.affiliation.other HMS^Orthopedic Surgery- Beth Israel-Deaconess en_US

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