Lassa Hemorrhagic Fever in a Late Term Pregnancy from Northern Sierra Leone with a Positive Maternal Outcome: Case Report

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Lassa Hemorrhagic Fever in a Late Term Pregnancy from Northern Sierra Leone with a Positive Maternal Outcome: Case Report

Show simple item record Branco, Luis M. Boisen, Matt L. Andersen, Kristian G Grove, Jessica N. Moses, Lina M. Muncy, Ivana J. Henderson, Lee A. Schieffellin, John S. Robinson, James E. Bangura, James J. Grant, Donald S. Raabe, Vanessa N. Fonnie, Mbalu Sabeti, Pardis Christine Garry, Robert F. 2011-11-02T17:53:46Z 2011
dc.identifier.citation Branco, Luis M., Matt L. Boisen, Kristian G. Andersen, Jessica N. Grove, Lina M. Moses, Ivana J. Muncy, Lee A. Henderson et al. 2011. Lassa hemorrhagic fever in a late term pregnancy from northern sierra leone with a positive maternal outcome: case report. Virology Journal 8(1):404. en_US
dc.identifier.issn 1743-422X en_US
dc.description.abstract Lassa fever (LF) is a devastating viral disease prevalent in West Africa. Efforts to take on this public health crisis have been hindered by lack of infrastructure and rapid field deployable diagnosis in areas where the disease is prevalent. Recent capacity building at the Kenema Government Hospital Lassa Fever Ward (KGH LFW) in Sierra Leone has lead to a major turning point in the diagnosis, treatment and study of LF. Herein we present the first comprehensive rapid diagnosis and real time characterization of an acute hemorrhagic LF case at KGH LFW. This case report focuses on a third trimester pregnant Sierra Leonean woman from the historically non-endemic Northern district of Tonkolili who survived the illness despite fetal demise. Employed in this study were newly developed recombinant LASV Antigen Rapid Test cassettes and dipstick lateral flow immunoassays (LFI) that enabled the diagnosis of LF within twenty minutes of sample collection. Deregulation of overall homeostasis, significant hepatic and renal system involvement, and immunity profiles were extensively characterized during the course of hospitalization. Rapid diagnosis, prompt treatment with a full course of intravenous (IV) ribavirin, IV fluids management, and real time monitoring of clinical parameters resulted in a positive maternal outcome despite admission to the LFW seven days post onset of symptoms, fetal demise, and a natural still birth delivery. These studies solidify the growing rapid diagnostic, treatment, and surveillance capabilities at the KGH LF Laboratory, and the potential to significantly improve the current high mortality rate caused by LF. As a result of the growing capacity, we were also able to isolate Lassa virus (LASV) RNA from the patient and perform Sanger sequencing where we found significant genetic divergence from commonly circulating Sierra Leonean strains, showing potential for the discovery of a newly emerged LASV strain with expanded geographic distribution. Furthermore, recent emergence of LF cases in Northern Sierra Leone highlights the need for superior diagnostics to aid in the monitoring of LASV strain divergence with potentially increased geographic expansion. en_US
dc.description.sponsorship Organismic and Evolutionary Biology en_US
dc.description.sponsorship Other Research Unit en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/1743-422X-8-404 en_US
dc.relation.hasversion en_US
dash.license OAP
dc.subject West-Africa en_US
dc.subject virus en_US
dc.subject management en_US
dc.subject diagnosis en_US
dc.subject expression en_US
dc.subject infection en_US
dc.subject Germany en_US
dc.title Lassa Hemorrhagic Fever in a Late Term Pregnancy from Northern Sierra Leone with a Positive Maternal Outcome: Case Report en_US
dc.type Journal Article en_US
dc.description.version Accepted Manuscript en_US
dc.relation.journal Virology Journal en_US Sabeti, Pardis Christine 2011-11-02T17:53:46Z
dash.affiliation.other Broad Institute of MIT and Harvard University en_US
dash.affiliation.other Harvard School of Public Health, Department of Immunology and Infectious Diseases en_US

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