ScanImage: Flexible Software for Operating Laser Scanning Microscopes

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ScanImage: Flexible Software for Operating Laser Scanning Microscopes

Show simple item record Pologruto, Thomas A Svoboda, Karel Sabatini, Bernardo Luis 2011-11-29T05:03:39Z 2003
dc.identifier.citation Pologruto, Thomas A., Bernardo L. Sabatini, and Karel Svoboda. 2003. ScanImage: Flexible software for operating laser scanning microscopes. BioMedical Engineering OnLine 2: 13. en_US
dc.identifier.issn 1475-925X en_US
dc.description.abstract Background Laser scanning microscopy is a powerful tool for analyzing the structure and function of biological specimens. Although numerous commercial laser scanning microscopes exist, some of the more interesting and challenging applications demand custom design. A major impediment to custom design is the difficulty of building custom data acquisition hardware and writing the complex software required to run the laser scanning microscope.Results We describe a simple, software-based approach to operating a laser scanning microscope without the need for custom data acquisition hardware. Data acquisition and control of laser scanning are achieved through standard data acquisition boards. The entire burden of signal integration and image processing is placed on the CPU of the computer. We quantitate the effectiveness of our data acquisition and signal conditioning algorithm under a variety of conditions. We implement our approach in an open source software package (ScanImage) and describe its functionality.Conclusions We present ScanImage, software to run a flexible laser scanning microscope that allows easy custom design. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi: 10.1186/1475-925X-2-13 en_US
dc.relation.hasversion en_US
dash.license LAA
dc.title ScanImage: Flexible Software for Operating Laser Scanning Microscopes en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BioMedical Engineering OnLine en_US Sabatini, Bernardo Luis 2011-11-29T05:03:39Z
dash.affiliation.other HMS^Neurobiology en_US

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