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dc.contributor.authorRen, Tao
dc.contributor.authorZamboni, Dario S.
dc.contributor.authorRoy, Craig R.
dc.contributor.authorDietrich, William F.
dc.contributor.authorVance, Russell E.
dc.date.accessioned2011-11-29T07:01:46Z
dc.date.issued2006
dc.identifier.citationRen, Tao, Dario S. Zamboni, Craig R. Roy, William F. Dietrich, and Russell E. Vance. 2006. Flagellin-deficient legionella mutants evade caspase-1- and naip5-mediated macrophage immunity. PLoS Pathogens 2, no. 3: e18.en_US
dc.identifier.issn1553-7366en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:5355106
dc.description.abstractMacrophages from C57BL/6J (B6) mice restrict growth of the intracellular bacterial pathogen Legionella pneumophila. Restriction of bacterial growth requires caspase-1 and the leucine-rich repeat-containing protein Naip5 (Birc1e). We identified mutants of L. pneumophila that evade macrophage innate immunity. All mutants were deficient in expression of flagellin, the primary flagellar subunit, and failed to induce caspase-1-mediated macrophage death. Interestingly, a previously isolated flagellar mutant (fliI) that expresses, but does not assemble, flagellin did not replicate in macrophages, and induced macrophage death. Thus, flagellin itself, not flagella or motility, is required to initiate macrophage innate immunity. Immunity to Legionella did not require MyD88, an essential adaptor for toll-like receptor 5 (TLR5) signaling. Moreover, flagellin of Legionella and Salmonella induced cytotoxicity when delivered to the macrophage cytosol using Escherichia coli as a heterologous host. It thus appears that macrophages sense cytosolic flagellin via a TLR5-independent pathway that leads to rapid caspase-1-dependent cell death and provides defense against intracellular bacterial pathogens.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi://10.1371/journal.ppat.0020018en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1401497/pdf/en_US
dash.licenseLAA
dc.subjectimmunologyen_US
dc.subjectinfectious diseasesen_US
dc.subjectmicrobiologyen_US
dc.subjectmus (mouse)en_US
dc.subjecteubacteriaen_US
dc.titleFlagellin-Deficient Legionella Mutants Evade Caspase-1- and Naip5-Mediated Macrophage Immunityen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS Pathogensen_US
dash.depositing.authorRen, Tao
dc.date.available2011-11-29T07:01:46Z
dash.affiliation.otherHMS^Microbiology and Molecular Geneticsen_US
dc.identifier.doi10.1371/journal.ppat.0020018*
dash.contributor.affiliatedRen, Tao


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