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dc.contributor.authorDeleidi, Michela
dc.contributor.authorCooper, Oliver Jamie
dc.contributor.authorHargus, Gunnar
dc.contributor.authorLevy, Adam
dc.contributor.authorIsacson, Ole Stefan
dc.date.accessioned2011-12-01T19:15:59Z
dc.date.issued2011
dc.identifier.citationDeleidi, Michela, Oliver Cooper, Gunnar Hargus, Adam Levy, and Ole Isacson. 2011. Oct4-induced reprogramming is required for adult brain neural stem cell differentiation into midbrain dopaminergic neurons. PLoS ONE 6(5): e19926.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:5358874
dc.description.abstractNeural stem cells (NSCs) lose their competency to generate region-specific neuronal populations at an early stage during embryonic brain development. Here we investigated whether epigenetic modifications can reverse the regional restriction of mouse adult brain subventricular zone (SVZ) NSCs. Using a variety of chemicals that interfere with DNA methylation and histone acetylation, we showed that such epigenetic modifications increased neuronal differentiation but did not enable specific regional patterning, such as midbrain dopaminergic (DA) neuron generation. Only after Oct-4 overexpression did adult NSCs acquire a pluripotent state that allowed differentiation into midbrain DA neurons. DA neurons derived from Oct4-reprogrammed NSCs improved behavioural motor deficits in a rat model of Parkinson's disease (PD) upon intrastriatal transplantation. Here we report for the first time the successful differentiation of SVZ adult NSCs into functional region-specific midbrain DA neurons, by means of Oct-4 induced pluripotency.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0019926en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3104995/pdf/en_US
dash.licenseLAA
dc.subjectbiologyen_US
dc.subjectdevelopmental biologyen_US
dc.subjectstem cellsen_US
dc.subjectadult stem cellsen_US
dc.subjectinduced pluripotent stem cellsen_US
dc.subjectneural stem cellsen_US
dc.subjectneuroscienceen_US
dc.subjectneurobiology of disease and regenerationen_US
dc.subjectmedicineen_US
dc.subjectneurologyen_US
dc.subjectParkinson’s diseaseen_US
dc.titleOct4-Induced Reprogramming is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neuronsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorCooper, Oliver Jamie
dc.date.available2011-12-01T19:15:59Z
dash.affiliation.otherHMS^Psychiatry-McLean Hospitalen_US
dash.affiliation.otherHMS^Neurology-Massachusetts General Hospitalen_US
dc.identifier.doi10.1371/journal.pone.0019926*
dash.contributor.affiliatedIsacson, Ole
dash.contributor.affiliatedCooper, Oliver Jamie


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