Show simple item record

dc.contributor.authorDeliolanis, Nikolaos C.
dc.contributor.authorWurdinger, Thomas
dc.contributor.authorPike, Lisa
dc.contributor.authorTannous, Bakhos A.
dc.contributor.authorBreakefield, Xandra Owens
dc.contributor.authorWeissleder, Ralph
dc.contributor.authorNtziachristos, Vasilis
dc.date.accessioned2011-12-05T02:41:19Z
dc.date.issued2011
dc.identifier.citationDeliolanis, Nikolaos C., Thomas Wurdinger, Lisa Pike, Bakhos A. Tannous, Xandra O. Breakefield, Ralph Weissleder, and Vasilis Ntziachristos. 2011. In vivo tomographic imaging of red-shifted fluorescent proteins. Biomedical Optics Express 2(4): 887-900.en_US
dc.identifier.issn2156-7085en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:5360055
dc.description.abstractWe have developed a spectral inversion method for three-dimensional tomography of far-red and near-infrared fluorescent proteins in animals. The method was developed in particular to address the steep light absorption transition of hemoglobin from the visible to the far-red occurring around 600 nm. Using an orthotopic mouse model of brain tumors expressing the red-shifted fluorescent protein mCherry, we demonstrate significant improvements in imaging accuracy over single-wavelength whole body reconstructions. Furthermore, we show an improvement in sensitivity of at least an order of magnitude over green fluorescent protein (GFP) for whole body imaging. We discuss how additional sensitivity gains are expected with the use of further red-shifted fluorescent proteins and we explain the differences and potential advantages of this approach over two-dimensional planar imaging methods.en_US
dc.language.isoen_USen_US
dc.publisherOptical Society of Americaen_US
dc.relation.isversionofdoi:10.1364/BOE.2.000887en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072128/pdf/en_US
dash.licenseLAA
dc.titleIn Vivo Tomographic Imaging of Red-Shifted Fluorescent Proteinsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBiomedical Optics Expressen_US
dash.depositing.authorTannous, Bakhos A.
dc.date.available2011-12-05T02:41:19Z
dash.affiliation.otherHMS^Neurology-Massachusetts General Hospitalen_US
dash.affiliation.otherHMS^Neurology-Massachusetts General Hospitalen_US
dash.affiliation.otherHMS^Radiology-Massachusetts General Hospitalen_US
dash.affiliation.otherHMS^Systems Biologyen_US
dc.identifier.doi10.1364/BOE.2.000887*
dash.contributor.affiliatedBreakefield, Xandra
dash.contributor.affiliatedTannous, Bakhos
dash.contributor.affiliatedWeissleder, Ralph


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record