HURP Permits MTOC Sorting for Robust Meiotic Spindle Bipolarity, Similar to Extra Centrosome Clustering in Cancer Cells
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HURP Permits MTOC Sorting for Robust Meiotic Spindle Bipolarity, Similar to Extra Centrosome Clustering in Cancer Cells
| Title: | HURP Permits MTOC Sorting for Robust Meiotic Spindle Bipolarity, Similar to Extra Centrosome Clustering in Cancer Cells |
| Author: |
Breuer, Manuel; Kolano, Agnieszka; Li, Chao-Chin; Tsai, Ting-Fen; Kwon, Mijung; Pellman, David Steven; Verlhac, Marie-Helene; Brunet, Stephane
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Breuer, Manuel, Agnieszka Kolano, Mijung Kwon, Chao-Chin Li, Ting-Fen Tsai, David Pellman, Stephane Brunet, and Marie-Helene Verlhac. 2010. HURP permits MTOC sorting for robust meiotic spindle bipolarity, similar to extra centrosome clustering in cancer cells. Journal of Cell Biology 191(7): 1251-1260. |
| Full Text & Related Files: |
3010075.pdf (2.824Mb; PDF) 
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| Abstract: | In contrast to somatic cells, formation of acentriolar meiotic spindles relies on the organization of microtubules (MTs) and MT-organizing centers (MTOCs) into a stable bipolar structure. The underlying mechanisms are still unknown. We show that this process is impaired in hepatoma up-regulated protein (Hurp) knockout mice, which are viable but female sterile, showing defective oocyte divisions. HURP accumulates on interpolar MTs in the vicinity of chromosomes via Kinesin-5 activity. By promoting MT stability in the spindle central domain, HURP allows efficient MTOC sorting into distinct poles, providing bipolarity establishment and maintenance. Our results support a new model for meiotic spindle assembly in which HURP ensures assembly of a central MT array, which serves as a scaffold for the genesis of a robust bipolar structure supporting efficient chromosome congression. Furthermore, HURP is also required for the clustering of extra centrosomes before division, arguing for a shared molecular requirement of MTOC sorting in mammalian meiosis and cancer cell division. |
| Published Version: | doi:10.1083/jcb.201005065 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010075/pdf/ |
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| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:5360613 |
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