HURP Permits MTOC Sorting for Robust Meiotic Spindle Bipolarity, Similar to Extra Centrosome Clustering in Cancer Cells
MetadataShow full item record
CitationBreuer, Manuel, Agnieszka Kolano, Mijung Kwon, Chao-Chin Li, Ting-Fen Tsai, David Pellman, Stephane Brunet, and Marie-Helene Verlhac. 2010. HURP permits MTOC sorting for robust meiotic spindle bipolarity, similar to extra centrosome clustering in cancer cells. Journal of Cell Biology 191(7): 1251-1260.
AbstractIn contrast to somatic cells, formation of acentriolar meiotic spindles relies on the organization of microtubules (MTs) and MT-organizing centers (MTOCs) into a stable bipolar structure. The underlying mechanisms are still unknown. We show that this process is impaired in hepatoma up-regulated protein (Hurp) knockout mice, which are viable but female sterile, showing defective oocyte divisions. HURP accumulates on interpolar MTs in the vicinity of chromosomes via Kinesin-5 activity. By promoting MT stability in the spindle central domain, HURP allows efficient MTOC sorting into distinct poles, providing bipolarity establishment and maintenance. Our results support a new model for meiotic spindle assembly in which HURP ensures assembly of a central MT array, which serves as a scaffold for the genesis of a robust bipolar structure supporting efficient chromosome congression. Furthermore, HURP is also required for the clustering of extra centrosomes before division, arguing for a shared molecular requirement of MTOC sorting in mammalian meiosis and cancer cell division.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:5360613
- HMS Scholarly Articles