CD1c Bypasses Lysosomes to Present a Lipopeptide Antigen with 12 Amino Acids

View/ Open
Author
De Jong, Annemieke
Vazquez, Jenny
Cheng, Tan-Yun
Barral, Duarte C.
León, Luis
Riese, Richard
Costello, Catherine E.
Porcelli, Steven A.
Briken, Volker
Young, David C.
O'Connor, Peter B.
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1084/jem.20082480Metadata
Show full item recordCitation
Van Rhijn, Ildiko, David C. Young, Annemieke De Jong, Jenny Vazquez, Tan-Yun Cheng, Rahul Talekar, Duarte C. Barral, et al. 2009. CD1c bypasses lysosomes to present a lipopeptide antigen with 12 amino acids. The Journal of Experimental Medicine 206(6): 1409-1422.Abstract
The recent discovery of dideoxymycobactin (DDM) as a ligand for CD1a demonstrates how a nonribosomal lipopeptide antigen is presented to T cells. DDM contains an unusual acylation motif and a peptide sequence present only in mycobacteria, but its discovery raises the possibility that ribosomally produced viral or mammalian proteins that commonly undergo lipidation might also function as antigens. To test this, we measured T cell responses to synthetic acylpeptides that mimic lipoproteins produced by cells and viruses. CD1c presented an N-acyl glycine dodecamer peptide (lipo-12) to human T cells, and the response was specific for the acyl linkage as well as the peptide length and sequence. Thus, CD1c represents the second member of the CD1 family to present lipopeptides. lipo-12 was efficiently recognized when presented by intact cells, and unlike DDM, it was inactivated by proteases and augmented by protease inhibitors. Although lysosomes often promote antigen presentation by CD1, rerouting CD1c to lysosomes by mutating CD1 tail sequences caused reduction in lipo-12 presentation. Thus, although certain antigens require antigen processing in lysosomes, others are destroyed there, providing a hypothesis for the evolutionary conservation of large CD1 families containing isoforms that survey early endosomal pathways.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2715062/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:5978709
Collections
- HMS Scholarly Articles [15665]
Contact administrator regarding this item (to report mistakes or request changes)