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dc.contributor.authorNehme, Nadine T.
dc.contributor.authorQuintin, Jessica
dc.contributor.authorCho, Ju Hyun
dc.contributor.authorLee, Jeannie T.
dc.contributor.authorLafarge, Marie-Celine
dc.contributor.authorKocks, Christine
dc.contributor.authorFerrandon, Dominique
dc.date.accessioned2011-12-30T02:18:00Z
dc.date.issued2011
dc.identifier.citationNehme, Nadine T., Jessica Quintin, Ju Hyun Cho, Janice Lee, Marie-Celine Lafarge, Christine Kocks, and Dominique Ferrandon. 2011. Relative roles of the cellular and humoral responses in the Drosophila host defense against three gram-positive bacterial infections. PLoS ONE 6(3): e14743.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:5978717
dc.description.abstractBackground: Two NF-kappaB signaling pathways, Toll and immune deficiency (imd), are required for survival to bacterial infections in Drosophila. In response to septic injury, these pathways mediate rapid transcriptional activation of distinct sets of effector molecules, including antimicrobial peptides, which are important components of a humoral defense response. However, it is less clear to what extent macrophage-like hemocytes contribute to host defense. Methodology/Principal Findings: In order to dissect the relative importance of humoral and cellular defenses after septic injury with three different Gram-positive bacteria (Micrococcus luteus, Enterococcus faecalis, Staphylococcus aureus), we used latex bead pre-injection to ablate macrophage function in flies wildtype or mutant for various Toll and imd pathway components. We found that in all three infection models a compromised phagocytic system impaired fly survival – independently of concomitant Toll or imd pathway activation. Our data failed to confirm a role of the PGRP-SA and GNBP1 Pattern Recognition Receptors for phagocytosis of S. aureus. The Drosophila scavenger receptor Eater mediates the phagocytosis by hemocytes or S2 cells of E. faecalis and S. aureus, but not of M. luteus. In the case of M. luteus and E. faecalis, but not S. aureus, decreased survival due to defective phagocytosis could be compensated for by genetically enhancing the humoral immune response. Conclusions/Significance: Our results underscore the fundamental importance of both cellular and humoral mechanisms in Drosophila immunity and shed light on the balance between these two arms of host defense depending on the invading pathogen.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0014743en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048390/pdf/en_US
dash.licenseLAA
dc.subjectimmune responseen_US
dc.subjectinnate immunityen_US
dc.subjectbacterial infectionsen_US
dc.titleRelative Roles of the Cellular and Humoral Responses in the Drosophila Host Defense Against Three Gram-Positive Bacterial Infectionsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorLee, Jeannie T.
dc.date.available2011-12-30T02:18:00Z
dash.affiliation.otherHMS^Geneticsen_US
dc.identifier.doi10.1371/journal.pone.0014743*
dash.contributor.affiliatedKocks, Christine
dash.contributor.affiliatedLee, Jeannie


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