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dc.contributor.authorBollati, Valentina
dc.contributor.authorMarinelli, Barbara
dc.contributor.authorApostoli, Pietro
dc.contributor.authorBonzini, Matteo
dc.contributor.authorNordio, Francesco
dc.contributor.authorHoxha, Mirjam
dc.contributor.authorPegoraro, Valeria
dc.contributor.authorTarantini, Letizia
dc.contributor.authorCantone, Laura
dc.contributor.authorBertazzi, Pier Alberto
dc.contributor.authorMotta, Valeria
dc.contributor.authorSchwartz, Joel David
dc.contributor.authorBaccarelli, Andrea
dc.date.accessioned2012-01-03T03:00:16Z
dc.date.issued2010
dc.identifier.citationBollati, Valentina, Barbara Marinelli, Pietro Apostoli, Matteo Bonzini, Francesco Nordio, Mirjam Hoxha, Valeria Pegoraro, et al. 2010. Exposure to metal-rich particulate matter modifies the expression of candidate micrornas in peripheral blood leukocytes. Environmental Health Perspectives 118(6): 763-768.en_US
dc.identifier.issn0091-6765en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:5978741
dc.description.abstractBackground: Altered patterns of gene expression mediate the effects of particulate matter (PM) on human health, but mechanisms through which PM modifies gene expression are largely undetermined. MicroRNAs (miRNAs) are highly conserved, noncoding small RNAs that regulate the expression of broad gene networks at the posttranscriptional level. Objectives: We evaluated the effects of exposure to PM and PM metal components on candidate miRNAs (miR-222, miR-21, and miR-146a) related with oxidative stress and inflammatory processes in 63 workers at an electric-furnace steel plant. Methods: We measured miR-222, miR-21, and miR-146a expression in blood leukocyte RNA on the first day of a workweek (baseline) and after 3 days of work (postexposure). Relative expression of miRNAs was measured by real-time polymerase chain reaction. We measured blood oxidative stress (8-hydroxyguanine) and estimated individual exposures to PM\(_{1}\)PM\(_{1}\) (< 1 μm in aerodynamic diameter), PM\(_{10}\) (< 10 μm in aerodynamic diameter), coarse PM (PM\(_{10}\) minus PM\(_{1}\)), and PM metal components (chromium, lead, cadmium, arsenic, nickel, manganese) between the baseline and postexposure measurements. Results: Expression of miR-222 and miR-21 (using the 2\(^{−ΔΔCT}\) method) was significantly increased in postexposure samples (miR-222: baseline = 0.68 ± 3.41, postexposure = 2.16 ± 2.25, p = 0.002; miR-21: baseline = 4.10 ± 3.04, postexposure = 4.66 ± 2.63, p = 0.05). In postexposure samples, miR-222 expression was positively correlated with lead exposure (β = 0.41, p = 0.02), whereas miR-21 expression was associated with blood 8-hydroxyguanine (β = 0.11, p = 0.03) but not with individual PM size fractions or metal components. Postexposure expression of miR-146a was not significantly different from baseline (baseline = 0.61 ± 2.42, postexposure = 1.90 ± 3.94, p = 0.19) but was negatively correlated with exposure to lead (β = −0.51, p = 0.011) and cadmium (β = −0.42, p = 0.04). Conclusions: Changes in miRNA expression may represent a novel mechanism mediating responses to PM and its metal components.en_US
dc.language.isoen_USen_US
dc.publisherNational Institute of Environmental Health Sciencesen_US
dc.relation.isversionofdoi://10.1289/ehp.0901300en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898851/pdf/en_US
dash.licenseLAA
dc.subjectepigeneticsen_US
dc.subjectetiologyen_US
dc.subjectmiRNA expressionen_US
dc.subjectparticulate matteren_US
dc.subjectperipheral blood leukocytesen_US
dc.titleExposure to Metal-rich Particulate Matter Modifies the Expression of Candidate Micrornas in Peripheral Blood Leukocytesen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalEnvironmental Health Perspectivesen_US
dash.depositing.authorMotta, Valeria
dc.date.available2012-01-03T03:00:16Z
dash.affiliation.otherHMS^Medicine-Brigham and Women's Hospitalen_US
dash.affiliation.otherSPH^Exposure Epidemiology and Risk Programen_US
dash.affiliation.otherSPH^Exposure Epidemiology and Risk Programen_US
dc.identifier.doi10.1289/ehp.0901300*
dash.authorsorderedfalse
dash.contributor.affiliatedMotta, Valeria
dash.contributor.affiliatedBaccarelli, Andrea
dash.contributor.affiliatedSchwartz, Joel
dc.identifier.orcid0000-0002-3436-0640
dc.identifier.orcid0000-0002-2557-150X


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