Differential hRad17 Expression by Histologic Subtype of Ovarian Cancer
Young, Jennifer L
Koon, E Colin
Mok, Samuel C
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CitationYoung, Jennifer L, E Colin Koon, Joseph Kwong, William R Welch, Michael G Muto, Ross S Berkowitz, and Samuel C Mok. 2011. Differential hRad17 expression by histologic subtype of ovarian cancer. Journal of Ovarian Research 4:6.
AbstractBackground: In the search for unique ovarian cancer biomarkers, ovarian specific cDNA microarray analysis identified hRad17, a cell cycle checkpoint protein, as over-expressed in ovarian cancer. The aim of this study was to validate this expression. Methods: Immunohistochemistry was performed on 72 serous, 19 endometrioid, 10 clear cell, and 6 mucinous ovarian cancers, 9 benign ovarian tumors, and 6 normal ovarian tissue sections using an anti-hRad17 antibody. Western blot analysis and quantitative PCR were performed using cell lysates and total RNA prepared from 17 ovarian cancer cell lines and 6 normal ovarian epithelial cell cultures (HOSE). Results: Antibody staining confirmed upregulation of hRad17 in 49.5% of ovarian cancer cases. Immunohistochemistry demonstrated that only 42% of serous and 47% of endometrioid subtypes showed overexpression compared to 80% of clear cell and 100% of mucinous cancers. Western blot confirmed overexpression of hRad17 in cancer cell lines compared to HOSE. Quantitative PCR demonstrated an upregulation of hRad17 RNA by 1.5-7 fold. hRad17 RNA expression differed by subtype. Conclusions: hRad17 is over-expressed in ovarian cancer. This over-expression varies by subtype suggesting a role in the pathogenesis of these types. Functional studies are needed to determine the potential role of this protein in ovarian cancer.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:5978750
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