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dc.contributor.authorEtzkorn, Manuel
dc.contributor.authorBockmann, Anja
dc.contributor.authorBaldus, Marc
dc.date.accessioned2012-01-09T18:00:32Z
dc.date.issued2011
dc.identifier.citationEtzkorn, Manuel, Anja Bockmann, and Marc Baldus. 2011. Kinetic analysis of protein aggregation monitored by real-time 2D solid-state NMR spectroscopy. Journal of Biomolecular NMR 49(2): 121-129.en_US
dc.identifier.issn0925-2738en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:7349755
dc.description.abstractIt is shown that real-time 2D solid-state NMR can be used to obtain kinetic and structural information about the process of protein aggregation. In addition to the incorporation of kinetic information involving intermediate states, this approach can offer atom-specific resolution for all detectable species. The analysis was carried out using experimental data obtained during aggregation of the 10.4 kDa Crh protein, which has been shown to involve a partially unfolded intermediate state prior to aggregation. Based on a single real-time 2D \(^{13}\textrm{C}–^{13}\textrm{C}\) transition spectrum, kinetic information about the refolding and aggregation step could be extracted. In addition, structural rearrangements associated with refolding are estimated and several different aggregation scenarios were compared to the experimental data.en_US
dc.language.isoen_USen_US
dc.publisherSpringer Netherlandsen_US
dc.relation.isversionofdoi:10.1007/s10858-011-9468-6en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3042102/pdf/en_US
dash.licenseLAA
dc.subjectaggregationen_US
dc.subjectkineticen_US
dc.subjectsolid-state NMRen_US
dc.subjectreal-time spectroscopyen_US
dc.subjectCrhen_US
dc.titleKinetic Analysis of Protein Aggregation Monitored by Real-Time 2D Solid-State NMR Spectroscopyen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalJournal of Biomolecular NMRen_US
dash.depositing.authorEtzkorn, Manuel
dc.date.available2012-01-09T18:00:32Z
dash.affiliation.other100160en_US
dc.identifier.doi10.1007/s10858-011-9468-6*
dash.contributor.affiliatedEtzkorn, Manuel


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