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dc.contributor.authorArnaout, Ramy
dc.date.accessioned2012-01-22T02:19:29Z
dc.date.issued2005
dc.identifier.citationArnaout, Ramy A. 2005. Specificity and overlap in gene segment-defined antibody repertoires. BMC Genomics 6: 148.en_US
dc.identifier.issn1471-2164en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:8000931
dc.description.abstractBackground: To date several studies have sought to catalog the full suite of antibodies that humans naturally produce against single antigens or other specificities (repertoire). Here we analyze the properties of all sequenced repertoires in order to better understand the specificity of antibody responses. Specifically, we ask whether the large-scale sequencing of antibody repertoires might provide a diagnostic tool for detecting antigen exposure. We do this by examining the overlap in V\(_{H-}\), D\(_-\), and J\(_{H-}\) segment usage among sequenced repertoires. Results: We find that repertoire overlap in V\(_{H-}\), D\(_-\), and J\(_{H-}\) segment use is least for V\(_H\) segments and greatest for J\(_H\) segments, consistent with there being more V\(_H\) than J\(_H\) segments in the human genome. We find that for any two antigens chosen at random, chances are 90 percent that their repertoires' V\(_H\) segments will overlap by less than half, and 98 percent that their VDJ\(_H\) combinations will overlap by ≤10 percent. We ran computer simulations to test whether enrichment for specific VDJ\(_H\) combinations could be detected in "antigen-exposed" populations, and found that enrichment is detectable with moderate-to-high sensitivity and high specificity, even when some VDJ\(_H\) combinations are not represented at all in some test sets. Conclusion: Thus, as large-scale sequencing becomes cost-effective for clinical testing, we suggest that sequencing an individual's expressed antibody repertoire has the potential to become a useful diagnostic modality.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi://10.1186/1471-2164-6-148en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1277825/pdf/en_US
dash.licenseLAA
dc.titleSpecificity and Overlap in Gene Segment-defined Antibody Repertoiresen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBMC Genomicsen_US
dash.depositing.authorArnaout, Ramy
dc.date.available2012-01-22T02:19:29Z
dash.affiliation.otherHMS^Pathologyen_US
dc.identifier.doi10.1186/1471-2164-6-148*
dash.contributor.affiliatedArnaout, Ramy


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