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dc.contributor.authorLee, Chung-Sheng
dc.contributor.authorDias, Anusha P.
dc.contributor.authorJedrychowski, Mark P.
dc.contributor.authorPatel, Arvind H.
dc.contributor.authorHsu, Jeanne L.
dc.contributor.authorReed, Robin Elizabeth
dc.date.accessioned2012-01-30T03:53:47Z
dc.date.issued2008
dc.identifier.citationLee, Chung-Sheng, Anusha P. Dias, Mark Jedrychowski, Arvind H. Patel, Jeanne L. Hsu, and Robin Reed. 2008. Human DDX3 functions in translation and interacts with the translation initiation factor eIF3. Nucleic Acids Research 36(14): 4708-4718.en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:8063396
dc.description.abstractThe conserved RNA helicase DDX3 is of major medical importance due to its involvement in numerous cancers, human hepatitis C virus (HCV) and HIV. Although DDX3 has been reported to have a wide variety of cellular functions, its precise role remains obscure. Here, we raised a new antibody to DDX3 and used it to show that DDX3 is evenly distributed throughout the cytoplasm at steady state. Consistent with this observation, HA-tagged DDX3 also localizes to the cytoplasm. RNAi of DDX3 in both human and Drosophila cells shows that DDX3 is required for cell viability. Moreover, using RNAi, we show that DDX3 is required for expression of protein from reporter constructs. In contrast, we did not detect a role for DDX3 in nuclear steps in gene expression. Further insight into the function of DDX3 came from the observation that its major interaction partner is the multi-component translation initiation factor eIF3. We conclude that a primary function for DDX3 is in protein translation, via an interaction with eIF3.en_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.isversionofdoi: 10.1093/nar/gkn454en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504307/pdf/en_US
dash.licenseLAA
dc.titleHuman DDX3 Functions in Translation and Interacts with the Translation Initiation Factor eIF3en_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalNucleic Acids Researchen_US
dash.depositing.authorJedrychowski, Mark P.
dc.date.available2012-01-30T03:53:47Z
dash.affiliation.otherHMS^Cell Biologyen_US
dc.identifier.doi10.1093/nar/gkn454*
dash.contributor.affiliatedReed, Robin
dash.contributor.affiliatedJedrychowski, Mark


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