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dc.contributor.authorBollati, Valentina
dc.contributor.authorTarantini, Letizia
dc.contributor.authorHu, Howard
dc.contributor.authorSchwartz, Joel David
dc.contributor.authorWright, Rosalind Jo
dc.contributor.authorPark, Sung Kyun
dc.contributor.authorSparrow, David
dc.contributor.authorVokonas, Pantel S
dc.contributor.authorBaccarelli, Andrea
dc.contributor.authorWright, Robert O.
dc.date.accessioned2012-02-12T05:12:16Z
dc.date.issued2010
dc.identifier.citationWright, Robert O., Joel Schwartz, Rosalind J. Wright, Valentina Bollati, Letizia Tarantini, Sung Kyun Park, Howard Hu, David Sparrow, Pantel Vokonas, and Andrea Baccarelli. 2010. Biomarkers of lead exposure and DNA methylation within retrotransposons. Environmental Health Perspectives 118(6): 790-795.en_US
dc.identifier.issn0091-6765en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:8156575
dc.description.abstractBackground: DNA methylation is an epigenetic mark that regulates gene expression. Changes in DNA methylation within white blood cells may result from cumulative exposure to environmental metals such as lead. Bone lead, a marker of cumulative exposure, may therefore better predict DNA methylation than does blood lead. Objective: In this study we compared associations between lead biomarkers and DNA methylation. Methods: We measured global methylation in participants of the Normative Aging Study (all men) who had archived DNA samples. We measured patella and tibia lead levels by K-X-Ray fluorescence and blood lead by atomic absorption spectrophotometry. DNA samples from blood were used to determine global methylation averages within CpG islands of long interspersed nuclear elements-1 (LINE-1) and Alu retrotransposons. A mixed-effects model using repeated measures of Alu or LINE-1 as the dependent variable and blood/bone lead (tibia or patella in separate models) as the primary exposure marker was fit to the data. Results: Overall mean global methylation (± SD) was 26.3 ± 1.0 as measured by Alu and 76.8 ± 1.9 as measured by LINE-1. In the mixed-effects model, patella lead levels were inversely associated with LINE-1 (β = −0.25; p less than 0.01) but not Alu (β = −0.03; p = 0.4). Tibia lead and blood lead did not predict global methylation for either Alu or LINE-1. Conclusion: Patella lead levels predicted reduced global DNA methylation within LINE-1 elements. The association between lead exposure and LINE-1 DNA methylation may have implications for the mechanisms of action of lead on health outcomes, and also suggests that changes in DNA methylation may represent a biomarker of past lead exposure.en_US
dc.language.isoen_USen_US
dc.publisherNational Institute of Environmental Health Sciencesen_US
dc.relation.isversionofdoi:10.1289/ehp.0901429en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898855/pdf/en_US
dash.licenseLAA
dc.subjectagingen_US
dc.subjectDNA methylationen_US
dc.subjectepigeneticsen_US
dc.subjectleaden_US
dc.subjectmetalsen_US
dc.titleBiomarkers of Lead Exposure and DNA Methylation within Retrotransposonsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalEnvironmental Health Perspectivesen_US
dash.depositing.authorWright, Robert O.
dc.date.available2012-02-12T05:12:16Z
dash.affiliation.otherHMS^Medicine-Brigham and Women's Hospitalen_US
dash.affiliation.otherSPH^Environmental+Occupational Medicine+Epien_US
dash.affiliation.otherHMS^Pediatrics-Children's Hospitalen_US
dash.affiliation.otherHMS^Medicine-Brigham and Women's Hospitalen_US
dash.affiliation.otherSPH^Exposure Epidemiology and Risk Programen_US
dash.affiliation.otherHMS^Medicine-Brigham and Women's Hospitalen_US
dash.affiliation.otherSPH^Environmental+Occupational Medicine+Epien_US
dash.affiliation.otherSPH^Exposure Epidemiology and Risk Programen_US
dc.identifier.doi10.1289/ehp.0901429*
dash.authorsorderedfalse
dash.contributor.affiliatedWright, Rosalind Jo
dash.contributor.affiliatedVokonas, Pantel
dash.contributor.affiliatedWright, Robert
dash.contributor.affiliatedSparrow, David
dash.contributor.affiliatedBaccarelli, Andrea
dash.contributor.affiliatedSchwartz, Joel
dc.identifier.orcid0000-0002-3436-0640
dc.identifier.orcid0000-0002-2557-150X


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