Involvement of Arabidopsis RACK1 in Protein Translation and Its Regulation by Abscisic Acid

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Involvement of Arabidopsis RACK1 in Protein Translation and Its Regulation by Abscisic Acid

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dc.contributor.author Valerius, Oliver
dc.contributor.author Hall, Hardy
dc.contributor.author Zeng, Qingning
dc.contributor.author Li, Jian-Feng
dc.contributor.author Weston, David J.
dc.contributor.author Ellis, Brian E.
dc.contributor.author Chen, Jin-Gui
dc.contributor.author Guo, Jianjun
dc.contributor.author Wang, Shuqi
dc.date.accessioned 2012-03-12T04:01:57Z
dc.date.issued 2010
dc.identifier.citation Guo, Jianjun, Shucai Wang, Oliver Valerius, Hardy Hall, Qingning Zeng, Jian-Feng Li, David J. Weston, Brian E. Ellis, and Jin-Gui Chen. 2011. Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid. Plant Physiology 155(1): 370-383. en_US
dc.identifier.issn 0032-0889 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:8347353
dc.description.abstract Earlier studies have shown that RACK1 functions as a negative regulator of abscisic acid (ABA) responses in Arabidopsis (Arabidopsis thaliana), but the molecular mechanism of the action of RACK1 in these processes remains elusive. Global gene expression profiling revealed that approximately 40% of the genes affected by ABA treatment were affected in a similar manner by the rack1 mutation, supporting the view that RACK1 is an important regulator of ABA responses. On the other hand, coexpression analysis revealed that more than 80% of the genes coexpressed with RACK1 encode ribosome proteins, implying a close relationship between RACK1’s function and the ribosome complex. These results implied that the regulatory role for RACK1 in ABA responses may be partially due to its putative function in protein translation, which is one of the major cellular processes that mammalian and Saccharomyces cerevisiae RACK1 is involved in. Consistently, all three Arabidopsis RACK1 homologous genes, namely RACK1A, RACK1B, and RACK1C, complemented the growth defects of the S. cerevisiae cross pathway control2/rack1 mutant. In addition, RACK1 physically interacts with Arabidopsis Eukaryotic Initiation Factor6 (eIF6), whose mammalian homolog is a key regulator of 80S ribosome assembly. Moreover, rack1 mutants displayed hypersensitivity to anisomycin, an inhibitor of protein translation, and displayed characteristics of impaired 80S functional ribosome assembly and 60S ribosomal subunit biogenesis in a ribosome profiling assay. Gene expression analysis revealed that ABA inhibits the expression of both RACK1 and eIF6. Taken together, these results suggest that RACK1 may be required for normal production of 60S and 80S ribosomes and that its action in these processes may be regulated by ABA. en_US
dc.language.iso en_US en_US
dc.publisher American Society of Plant Biologists en_US
dc.relation.isversionof doi:10.1104/pp.110.160663 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3075769/pdf/ en_US
dash.license LAA
dc.subject Article en_US
dc.title Involvement of Arabidopsis RACK1 in Protein Translation and Its Regulation by Abscisic Acid en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Plant Physiology en_US
dash.depositing.author Guo, Jianjun
dc.date.available 2012-03-12T04:01:57Z
dash.affiliation.other 100157 en_US
dash.affiliation.other 100178 en_US

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