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dc.contributor.authorMehdiabadi, Natasha J
dc.contributor.authorKronforst, Marcus
dc.contributor.authorQueller, David C
dc.contributor.authorStrassmann, Joan E
dc.date.accessioned2012-03-13T16:08:37Z
dc.date.issued2010
dc.identifier.citationMehdiabadi, Natasha J., Marcus R. Kronforst, David C. Queller, and Joan E. Strassmann. 2010. Phylogeography and sexual macrocyst formation in the social amoeba \(Dictyostelium giganteum\). BMC Evolutionary Biology 10: 17.en_US
dc.identifier.issn1471-2148en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:8360414
dc.description.abstractBackground: Microorganisms are ubiquitous, yet we are only beginning to understand their diversity and population structure. Social amoebae (Dictyostelia) are a diverse group of unicellular eukaryotic microbes that display a unique social behaviour upon starvation in which cells congregate and then some die to help others survive and disperse. The genetic relationships among co-occurring cells have a major influence on the evolution of social traits and recent population genetic analysis found extensive genetic variation and possible cryptic speciation in one dictyostelid species \((Dictyostelium purpureum)\). To further characterize the interplay among genetic variation, species boundaries, social behaviour, and reproductive isolation in the Dictyostelia, we conducted phylogenetic analyses and mating experiments with the geographically widespread social amoeba \(Dictyostelium giganteum\). Results: We sequenced approximately 4,000 basepairs of the nuclear ribosomal DNA from 24 isolates collected from Texas, Michigan, Massachusetts, Virginia, and Wisconsin and identified 16 unique haplotypes. Analyses of the sequence data revealed very little genetic differentiation among isolates and no clear evidence of phylogenetic structure, although there was evidence for some genetic differentiation between the Massachusetts and Texas populations. These results suggest that sexual mating (macrocyst formation) is not likely to correlate with either genetic or geographical distance. To test this prediction, we performed 108 mating experiments and found no association between mating probability and genetic or geographical distance. Conclusions: \(D. giganteum\) isolates from across North America display little genetic variation, phylogeographic structure, and genetic differentiation among populations relative to the cryptic species observed within \(D. purpureum\). Furthermore, variation that does exist does not predict the probability of mating among clones. These results have important implications for our understanding of speciation and social evolution in microbes.en_US
dc.description.sponsorshipOther Research Uniten_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofhttp://www.biomedcentral.com/1471-2148/10/17en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2824659/pdf/en_US
dash.licenseOAP
dc.titlePhylogeography and Sexual Macrocyst Formation in the Social Amoeba \(Dictyostelium Giganteum\)en_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBMC Evolutionary Biologyen_US
dash.depositing.authorKronforst, Marcus
dc.date.available2012-03-13T16:08:37Z
dc.identifier.doi10.1186/1471-2148-10-17
dash.contributor.affiliatedKronforst, Marcus


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