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dc.contributor.authorHo, On
dc.contributor.authorLarsen, Kay
dc.contributor.authorPolacino, Patricia
dc.contributor.authorLi, Yun
dc.contributor.authorAnderson, David
dc.contributor.authorSong, Ruijiang
dc.contributor.authorRuprecht, Ruth Margrit
dc.contributor.authorHu, Shiu-Lok
dc.date.accessioned2012-03-23T14:08:37Z
dc.date.issued2009
dc.identifier.citationHo, On, Kay Larsen, Patricia Polacino, Yun Li, David Anderson, Ruijiang Song, Ruth M. Ruprecht, and Shiu-Lok Hu. 2009. Pathogenic infection of Macaca nemestrina with a CCR5-tropic subtype-C simian-human immunodeficiency virus. Retrovirology 6: 65.en_US
dc.identifier.issn1742-4690en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:8438165
dc.description.abstractBackground: Although pig-tailed macaques (Macaca nemestrina) have been used in AIDS research for years, less is known about the early immunopathogenic events in this species, as compared to rhesus macaques (Macaca mulatta). Similarly, the events in early infection are well-characterized for simian immunodeficiency viruses (SIV), but less so for chimeric simian-human immunodeficiency viruses (SHIV), although the latter have been widely used in HIV vaccine studies. Here, we report the consequences of intrarectal infection with a CCR5-tropic clade C SHIV-1157ipd3N4 in pig-tailed macaques. Results: Plasma and cell-associated virus was detectable in peripheral blood and intestinal tissues of all four pig-tailed macaques following intrarectal inoculation with SHIV-1157ipd3N4. We also observed a rapid and irreversible loss of CD4+ T cells at multiple mucosal sites, resulting in a marked decrease of CD4:CD8 T cell ratios 0.5–4 weeks after inoculation. This depletion targeted subsets of CD4+ T cells expressing the CCR5 coreceptor and having a CD28-CD95+ effector memory phenotype, consistent with the R5-tropism of SHIV-1157ipd3N4. All three animals that were studied beyond the acute phase seroconverted as early as week 4, with two developing cross-clade neutralizing antibody responses by week 24. These two animals also demonstrated persistent plasma viremia for >48 weeks. One of these animals developed AIDS, as shown by peripheral blood CD4+ T-cell depletion starting at 20 weeks post inoculation. Conclusion: These findings indicate that SHIV-1157ipd3N4-induced pathogenesis in pig-tailed macaques followed a similar course as SIV-infected rhesus macaques. Thus, R5 SHIV-C-infection of pig-tailed macaques could provide a useful and relevant model for AIDS vaccine and pathogenesis research.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/1742-4690-6-65en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720380/pdf/en_US
dash.licenseLAA
dc.titlePathogenic Infection of Macaca Nemestrina with a CCR5-tropic Subtype-C Simian-human Immunodeficiency Virusen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalRetrovirologyen_US
dash.depositing.authorRuprecht, Ruth Margrit
dc.date.available2012-03-23T14:08:37Z
dash.affiliation.otherHMS^Medicine-Brigham and Women's Hospitalen_US
dc.identifier.doi10.1186/1742-4690-6-65*
dash.contributor.affiliatedRuprecht, Ruth Margrit


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