Pathway Analysis Following Association Study
| Title: | Pathway Analysis Following Association Study |
| Author: |
Lu, Chen; Ngwa, Julius S.; Grimsby, Jonna L.; Zhuang, Wei V.; DeStefano, Anita L.; Manning, Alisa K.
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Ngwa, Julius S., Alisa K. Manning, Jonna L. Grimsby, Chen Lu, Wei V. Zhuang, and Anita L. DeStefano. 2011. Pathway analysis following association study. BMC Proceedings 5(Suppl 9): S18. |
| Full Text & Related Files: |
3287852.pdf (294.8Kb; PDF) 
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| Abstract: | Genome-wide association studies often emphasize single-nucleotide polymorphisms with the smallest p-values with less attention given to single-nucleotide polymorphisms not ranked near the top. We suggest that gene pathways contain valuable information that can enable identification of additional associations. We used gene set information to identify disease-related pathways using three methods: gene set enrichment analysis (GSEA), empirical enrichment p-values, and Ingenuity pathway analysis (IPA). Association tests were performed for common single-nucleotide polymorphisms and aggregated rare variants with traits Q1 and Q4. These pathway methods were evaluated by type I error, power, and the ranking of the VEGF pathway, the gene set used in the simulation model. GSEA and IPA had high power for detecting the VEGF pathway for trait Q1 (91.2% and 93%, respectively). These two methods were conservative with deflated type I errors (0.0083 and 0.0072, respectively). The VEGF pathway ranked 1 or 2 in 123 of 200 replicates using IPA and ranked among the top 5 in 114 of 200 replicates for GSEA. The empirical enrichment method had lower power and higher type I error. Thus pathway analysis approaches may be useful in identifying biological pathways that influence disease outcomes. |
| Published Version: | doi:10.1186/1753-6561-5-S9-S18 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287852 |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:8519648 |
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