Scavenger Receptors and \(\beta\)-Glucan Receptors Participate in the Recognition of Yeasts by Murine Macrophages

DSpace/Manakin Repository

Scavenger Receptors and \(\beta\)-Glucan Receptors Participate in the Recognition of Yeasts by Murine Macrophages

Citable link to this page

 

 
Title: Scavenger Receptors and \(\beta\)-Glucan Receptors Participate in the Recognition of Yeasts by Murine Macrophages
Author: Józefowski, Szczepan; Marcinkiewicz, Janusz; Yang, Zhiping; Kobzik, Lester

Note: Order does not necessarily reflect citation order of authors.

Citation: Józefowski, Szczepan, Zhiping Yang, Janusz Marcinkiewicz, and Lester Kobzik. 2012. Scavenger receptors and \(\beta\)-glucan receptors participate in the recognition of yeasts by murine macrophages. Inflammation Research 61(2): 113-126.
Full Text & Related Files:
Abstract: Objectives: Numerous receptors have been implicated in recognition of pathogenic fungi by macrophages, including the \(\beta\)-glucan receptor dectin-1. The role of scavenger receptors (SRs) in anti-fungal immunity is not well characterized. Methods: We studied uptake of unopsonized Saccharomycetes cerevisiae (zymosan) and live Candida albicans yeasts as well as zymosan-stimulated \(H_2O_2\) production in J774 macrophage-like cells and peritoneal exudate macrophages (PEMs). The role of different receptors was assessed with the use of competitive ligands, transfected cells and receptor-deficient macrophages. Results: The uptake of zymosan by untreated J774 cells was mediated approximately half by SRs and half by a \(\beta\)-glucan receptor which was distinct from dectin-1 and not linked to stimulation of \(H_2O_2\) production. Ligands of \(\beta\)-glucan receptors and of SRs also inhibited uptake of C. albicans by macrophages (J774 cells and PEMs). In macrophages pretreated with a CpG motif-containing oligodeoxynucleotide (CpG-ODN) the relative contribution of SRs to yeast uptake increased and that of \(\beta\)-glucan receptors decreased. Whereas the class A SR MARCO participated in the uptake of both zymosan and C. albicans by CpG-ODN-pretreated, but not untreated macrophages, the related receptor SR-A/CD204 was involved in the uptake of zymosan, but not of C. albicans. The reduction of zymosan-stimulated \(H_2O_2\) production observed in DS-pretreated J774 cells and in class A SRs-deficient PEMs suggest that class A SRs mediate part of this process. Conclusions: Our results revealed that SRs belong to a redundant system of receptors for yeasts. Binding of yeasts to different receptors in resting versus CpG-ODN-pre-exposed macrophages may differentially affect polarization of adaptive immune responses.
Published Version: doi:10.1007/s00011-011-0395-5
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265724/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:8605329
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters