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dc.contributor.authorBerde, Charles Benjamin
dc.contributor.authorAthiraman, Umeshkumar
dc.contributor.authorYahalom, Barak
dc.contributor.authorZurakowski, David
dc.contributor.authorCorfas, Gabriel
dc.contributor.authorBognet, Christina
dc.date.accessioned2012-04-25T17:44:31Z
dc.date.issued2011
dc.identifier.citationBerde, Charles B., Umeshkumar Athiraman, Barak Yahalom, David Zurakowski, Gabriel Corfas, and Christina Bognet. 2011. Tetrodotoxin-Bupivacaine-Epinephrine combinations for prolonged local anesthesia. Marine Drugs 9(12): 2717-2728.en_US
dc.identifier.issn1660-3397en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:8617188
dc.description.abstractCurrently available local anesthetics have analgesic durations in humans generally less than 12 hours. Prolonged-duration local anesthetics will be useful for postoperative analgesia. Previous studies showed that in rats, combinations of tetrodotoxin (TTX) with bupivacaine had supra-additive effects on sciatic block durations. In those studies, epinephrine combined with TTX prolonged blocks more than 10-fold, while reducing systemic toxicity. TTX, formulated as Tectin, is in phase III clinical trials as an injectable systemic analgesic for chronic cancer pain. Here, we examine dose-duration relationships and sciatic nerve histology following local nerve blocks with combinations of Tectin with bupivacaine 0.25% (2.5 mg/mL) solutions, with or without epinephrine 5 µg/mL (1:200,000) in rats. Percutaneous sciatic blockade was performed in Sprague-Dawley rats, and intensity and duration of sensory blockade was tested blindly with different Tectin-bupivacaine-epinephrine combinations. Between-group comparisons were analyzed using ANOVA and post-hoc Sidak tests. Nerves were examined blindly for signs of injury. Blocks containing bupivacaine 0.25% with Tectin 10 µM and epinephrine 5 µg/mL were prolonged by roughly 3-fold compared to blocks with bupivacaine 0.25% plain (P < 0.001) or bupivacaine 0.25% with epinephrine 5 µg/mL (P < 0.001). Nerve histology was benign for all groups. Combinations of Tectin in bupivacaine 0.25% with epinephrine 5 µg/mL appear promising for prolonged duration of local anesthesia.en_US
dc.language.isoen_USen_US
dc.publisherMolecular Diversity Preservation International (MDPI)en_US
dc.relation.isversionofdoi:10.3390/md9122717en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280572/pdf/en_US
dash.licenseLAA
dc.subjecttetrodotoxinen_US
dc.subjectbupivacaineen_US
dc.subjectepinephrineen_US
dc.subjectlocal anesthesiaen_US
dc.subjectsciatic blockadeen_US
dc.titleTetrodotoxin-Bupivacaine-Epinephrine Combinations for Prolonged Local Anesthesiaen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalMarine Drugsen_US
dash.depositing.authorCorfas, Gabriel
dc.date.available2012-04-25T17:44:31Z
dc.identifier.doi10.3390/md9122717*
dash.contributor.affiliatedYahalom, Barak
dash.contributor.affiliatedCorfas, Gabriel
dash.contributor.affiliatedBerde, Charles
dash.contributor.affiliatedZurakowski, David


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