dc.contributor.author | Berde, Charles Benjamin | |
dc.contributor.author | Athiraman, Umeshkumar | |
dc.contributor.author | Yahalom, Barak | |
dc.contributor.author | Zurakowski, David | |
dc.contributor.author | Corfas, Gabriel | |
dc.contributor.author | Bognet, Christina | |
dc.date.accessioned | 2012-04-25T17:44:31Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Berde, Charles B., Umeshkumar Athiraman, Barak Yahalom, David Zurakowski, Gabriel Corfas, and Christina Bognet. 2011. Tetrodotoxin-Bupivacaine-Epinephrine combinations for prolonged local anesthesia. Marine Drugs 9(12): 2717-2728. | en_US |
dc.identifier.issn | 1660-3397 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:8617188 | |
dc.description.abstract | Currently available local anesthetics have analgesic durations in humans generally less than 12 hours. Prolonged-duration local anesthetics will be useful for postoperative analgesia. Previous studies showed that in rats, combinations of tetrodotoxin (TTX) with bupivacaine had supra-additive effects on sciatic block durations. In those studies, epinephrine combined with TTX prolonged blocks more than 10-fold, while reducing systemic toxicity. TTX, formulated as Tectin, is in phase III clinical trials as an injectable systemic analgesic for chronic cancer pain. Here, we examine dose-duration relationships and sciatic nerve histology following local nerve blocks with combinations of Tectin with bupivacaine 0.25% (2.5 mg/mL) solutions, with or without epinephrine 5 µg/mL (1:200,000) in rats. Percutaneous sciatic blockade was performed in Sprague-Dawley rats, and intensity and duration of sensory blockade was tested blindly with different Tectin-bupivacaine-epinephrine combinations. Between-group comparisons were analyzed using ANOVA and post-hoc Sidak tests. Nerves were examined blindly for signs of injury. Blocks containing bupivacaine 0.25% with Tectin 10 µM and epinephrine 5 µg/mL were prolonged by roughly 3-fold compared to blocks with bupivacaine 0.25% plain (P < 0.001) or bupivacaine 0.25% with epinephrine 5 µg/mL (P < 0.001). Nerve histology was benign for all groups. Combinations of Tectin in bupivacaine 0.25% with epinephrine 5 µg/mL appear promising for prolonged duration of local anesthesia. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Molecular Diversity Preservation International (MDPI) | en_US |
dc.relation.isversionof | doi:10.3390/md9122717 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280572/pdf/ | en_US |
dash.license | LAA | |
dc.subject | tetrodotoxin | en_US |
dc.subject | bupivacaine | en_US |
dc.subject | epinephrine | en_US |
dc.subject | local anesthesia | en_US |
dc.subject | sciatic blockade | en_US |
dc.title | Tetrodotoxin-Bupivacaine-Epinephrine Combinations for Prolonged Local Anesthesia | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | Marine Drugs | en_US |
dash.depositing.author | Corfas, Gabriel | |
dc.date.available | 2012-04-25T17:44:31Z | |
dc.identifier.doi | 10.3390/md9122717 | * |
dash.contributor.affiliated | Yahalom, Barak | |
dash.contributor.affiliated | Corfas, Gabriel | |
dash.contributor.affiliated | Berde, Charles | |
dash.contributor.affiliated | Zurakowski, David | |