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dc.contributor.authorEgli, Dieter
dc.contributor.authorEggan, Kevin Carl
dc.date.accessioned2012-06-22T02:13:01Z
dc.date.issued2010
dc.identifier.citationEgli, Dieter, and Kevin Carl Eggan. 2010. Recipient cell nuclear factors are required for reprogramming by nuclear transfer. Development 137(12): 1953-1963.en_US
dc.identifier.issn0950-1991en_US
dc.identifier.issn1477-9129en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:8909917
dc.description.abstractNuclear transfer allows the reprogramming of somatic cells to totipotency. The cell cycle state of the donor and recipient cells, as well as their extent of differentiation, have each been cited as important determinants of reprogramming success. Here, we have used donor and recipient cells at various cell cycle and developmental stages to investigate the importance of these parameters. We found that many stages of the cell cycle were compatible with reprogramming as long as a sufficient supply of essential nuclear factors, such as Brg1, were retained in the recipient cell following enucleation. Consistent with this conclusion, the increased efficiency of reprogramming when using donor nuclei from embryonic cells could be explained, at least in part, by reintroduction of embryonic nuclear factors along with the donor nucleus. By contrast, cell cycle synchrony between the donor nucleus and the recipient cell was not required at the time of transfer, as long as synchrony was reached by the first mitosis. Our findings demonstrate the remarkable flexibility of the reprogramming process and support the importance of nuclear transcriptional regulators in mediating reprogramming.en_US
dc.description.sponsorshipMolecular and Cellular Biologyen_US
dc.language.isoen_USen_US
dc.publisherCompany of Biologistsen_US
dc.relation.isversionofdoi:10.1242/dev.046151en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pubmed/20463036en_US
dash.licenseOAP
dc.subjectnucleusen_US
dc.subjectmitosisen_US
dc.subjectnuclear envelopeen_US
dc.subjectreprogrammingen_US
dc.subjectdevelopmenten_US
dc.subjectBrg1en_US
dc.titleRecipient Cell Nuclear Factors Are Required for Reprogramming by Nuclear Transferen_US
dc.typeJournal Articleen_US
dc.description.versionAuthor's Originalen_US
dc.relation.journalDevelopmenten_US
dash.depositing.authorEggan, Kevin Carl
dc.date.available2012-06-22T02:13:01Z
dc.identifier.doi10.1242/dev.046151*
dash.contributor.affiliatedEggan, Kevin


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