Thermal and Solvent-Isotope Effects on the Flagellar Rotary Motor near Zero Load

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Thermal and Solvent-Isotope Effects on the Flagellar Rotary Motor near Zero Load

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Title: Thermal and Solvent-Isotope Effects on the Flagellar Rotary Motor near Zero Load
Author: Berg, Howard Curtis; Yuan, Junhua

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Citation: Yuan, Junhua and Howard C. Berg. 2010. Thermal and solvent-isotope effects on the flagellar rotary motor near zero load. Biophysical Journal 98(10): 2121-2126.
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Abstract: In Escherichia coli, the behavior of the flagellar rotary motor near zero load can be studied by scattering light from nanogold spheres attached to proximal hooks of cells lacking flagellar filaments. We used this method to monitor changes in speed when cells were subjected to changes in temperature or shifted from a medium made with \(H{_2}O\) to one made with \(D_{2}O\). In \(H{_2}O\), the speed increased with temperature in a near-exponential manner, with an activation enthalpy of \(52 \pm 4\) kJ/mol (\(12.0 \pm 1.0\) kcal/mol). In \(D_{2}O\), the speed increased in a similar manner, with an activation enthalpy of \(50 \pm 4\) kJ/mol. The speed in \(H{_2}O\) was higher than that in \(D_{2}O\) by a factor of \(1.53 \pm 0.14\). We performed comparison studies of variations in temperature and solvent isotope, using motors operating at high loads. The variations were small, consistent with previous observations. The implications of these results for proton translocation are discussed.
Published Version: doi: 10.1016/j.bpj.2010.01.061
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:9032268
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